Peb tau sib koom ntau qhov kev txiav txim siab lub lim tiam dhau los rau kev siv cellulose acetate membrane electrophoresis, thiab peb yuav ua tiav cov ntsiab lus ntawm no hnub no rau koj siv.
Kev xaiv ntawm Buffer Concentration
Qhov tsis pom kev siv hauv cellulose acetate membrane electrophoresis feem ntau qis dua li siv hauv daim ntawv electrophoresis.Feem ntau siv pH 8.6Barbital tsis yog feem ntau xaiv nyob rau hauv thaj tsam ntawm 0.05 mol / L txog 0.09 mol / L.Thaum xaiv qhov concentration, qhov kev txiav txim ua ntej yog ua.Piv txwv li, yog hais tias qhov ntev ntawm daim nyias nyias ntawm cov electrodes nyob rau hauv lub electrophoresis chamber yog 8-10cm, ib tug voltage ntawm 25V yuav tsum tau ib tug centimeter ntawm daim nyias nyias ntev, thiab tam sim no siv yuav tsum yog 0.4-0.5 mA ib centimeter ntawm daim nyias nyias.Yog tias cov txiaj ntsig no tsis ua tiav lossis ntau dhau thaum lub sijhawm electrophoresis, qhov tsis muaj concentration yuav tsum tau nce lossis diluted.
Ib qho tsis tshua muaj siab tsis txaus yuav ua rau muaj kev txav ceev ntawm cov bands thiab nce hauv band dav.Ntawm qhov tod tes, qhov tsis txaus siab ntau dhau yuav ua rau qeeb ntawm kev tsiv teb tsaws chaw, ua rau nws nyuaj rau kev paub qhov txawv ntawm qee qhov sib cais.
Nws yuav tsum tau muab sau tseg tias nyob rau hauv cellulose acetate membrane electrophoresis, ib feem tseem ceeb ntawm cov tam sim no yog ua los ntawm cov qauv, uas generates ib tug ntau npaum li cas ntawm tshav kub.Qee lub sij hawm, qhov kev xaiv tsis muaj qhov xav tau tuaj yeem suav tias tsim nyog.Txawm li cas los xij, nyob rau hauv cov xwm txheej ntawm qhov kub ntawm ib puag ncig los yog thaum siv hluav taws xob ntau dua, cov evaporation ntawm cov dej vim cua sov tuaj yeem ua rau muaj zog, ua rau muaj qhov tsis txaus siab ntau heev thiab ua rau cov membrane qhuav.
Qauv Volume
Nyob rau hauv cellulose acetate membrane electrophoresis, tus nqi ntawm cov qauv ntim yog txiav txim los ntawm ntau yam, nrog rau electrophoresis tej yam kev mob, cov khoom ntawm tus qauv nws tus kheej, staining txoj kev, thiab nrhiav kom tau cov tswv yim.Raws li lub hauv paus ntsiab lus dav dav, qhov kev nkag siab ntau dua qhov kev tshawb pom, qhov me me ntawm cov qauv ntim tuaj yeem ua tau, uas yog qhov zoo rau kev sib cais.Yog tias cov qauv ntim ntau dhau, cov qauv sib cais electrophoretic yuav tsis meej, thiab staining tuaj yeem siv sijhawm ntev.Txawm li cas los xij, thaum txheeb xyuas qhov sib cais ntawm cov kab sib cais siv cov txheej txheem elution colorimetric nrhiav kom pom, cov qauv ntim yuav tsum tsis txhob me me, vim tias nws tuaj yeem ua rau qis absorbance qhov tseem ceeb rau qee yam khoom, ua rau muaj kev ua yuam kev ntau dua hauv kev suav lawv cov ntsiab lus.Hauv cov xwm txheej zoo li no, tus qauv ntim yuav tsum tau nce kom tsim nyog.
Feem ntau, cov qauv ntim ntxiv ntawm txhua centimeter ntawm cov qauv daim ntawv thov kab yog li ntawm 0.1 txog 5 μL, sib npaug rau cov qauv ntawm 5 txog 1000 μg.Piv txwv li, nyob rau hauv niaj hnub serum protein electrophoresis tsom xam, cov qauv ntim ntxiv ntawm txhua centimeter ntawm daim ntawv thov kab feem ntau tsis tshaj 1 μL, sib npaug rau 60 mus rau 80 μg ntawm cov protein.Txawm li cas los xij, thaum soj ntsuam lipoproteins lossis glycoproteins siv tib txoj kev electrophoresis, cov qauv ntim yuav tsum tau nce ntxiv.
Hauv kev xaus, cov qauv tsim nyog tshaj plaws yuav tsum raug xaiv raws li cov xwm txheej tshwj xeeb los ntawm kev sim ua ntej.
Kev xaiv Staining Solution
Cov kab sib cais hauv cellulose acetate membrane electrophoresis feem ntau yog stained ua ntej kuaj pom.Cov qauv sib txawv yuav tsum muaj ntau txoj kev staining, thiab cov txheej txheem staining haum rau cellulose acetate membrane electrophoresis yuav tsis siv rau lim ntawv.
Muaj peb lub hauv paus ntsiab lus tseem ceeb los xaiv cov tshuaj pleev xim raucellulose acetate daim nyias nyias.Ua ntej,Cov dej-soluble dyes yuav tsum tau nyiam dua li cawv-soluble dyes kom tsis txhob muaj cov membrane shrinkage thiab deformation tshwm sim los ntawm tom kawg staining tov.Tom qab staining, nws yog ib qho tseem ceeb kom yaug lub membrane nrog dej thiab txo lub sij hawm staining.Txwv tsis pub, daim nyias nyias yuav ua curled los yog shrunken, uas yuav cuam tshuam rau kev tshawb nrhiav tom qab.
Qhov thib ob, nws yog qhov zoo dua los xaiv dyes nrog muaj zog staining affinity rau cov qauv.Nyob rau hauv cellulose acetate membrane electrophoresis ntawm cov ntshav cov protein, amino dub 10B feem ntau yog siv vim nws muaj zog staining affinity rau ntau yam serum protein Cheebtsam thiab nws stability.
Thirdly, txhim khu kev qha zoo dyes yuav tsum tau xaiv.Qee cov dyes, txawm tias muaj tib lub npe, tuaj yeem muaj cov impurities uas ua rau muaj qhov tsaus ntuj tshwj xeeb tom qab staining.Qhov no tuaj yeem ua rau qhov muag tsis pom qhov qub zoo sib cais, ua rau lawv nyuaj rau qhov txawv.
Thaum kawg, kev xaiv staining tov concentration yog qhov tseem ceeb.Raws li txoj cai, nws yuav zoo li tias ntau dua staining tov concentration yuav ua rau kom meej meej staining ntawm cov qauv thiab cov txiaj ntsig zoo dua.Txawm li cas los xij, qhov no tsis yog li ntawd.Qhov kev sib txuas ntawm cov khoom piv txwv thiab cov zas xim muaj qee qhov kev txwv, uas tsis nce nrog qhov nce hauv cov tshuaj staining concentration.Ntawm qhov tsis sib xws, qhov kev daws teeb meem ntau dhau los tsis yog tsuas yog khib nyiab cov xim xwb tab sis kuj ua rau nws nyuaj rau kev ua tiav cov keeb kwm meej.Ntxiv mus, thaum cov xim siv tau mus txog ib qho txiaj ntsig siab tshaj plaws, cov dye's absorbance nkhaus tsis ua raws li kev sib raug zoo, tshwj xeeb tshaj yog nyob rau hauv kev ntsuas ntau.
Paub meej txog Beijing Liuyi Biotechnology's cellulose acetate daim nyias nyiaselectrophoresis tank thiab nws daim ntawv thov electrophoresis, thov mus saib ntawm no:
lKev sim rau kev cais cov ntshav protein los ntawm Cellulose Acetate Membrane
lCellulose Acetate Membrane Electrophoresis
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb.Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Siv:Electrophoresis (ib tsab thib ob) los ntawm Mr. Li
Post lub sij hawm: Jun-06-2023