Protein Electrophoresis Common Issues

Protein electrophoresis band teeb meem hais txog cov teeb meem lossis qhov tsis sib xws uas yuav tshwm sim thaum lub sij hawm sib cais cov proteins raws li lawv cov nqi hluav taws xob siv electrophoresis.Cov teeb meem no tuaj yeem suav nrog qhov pom ntawm qhov kev npaj txhij txog lossis txawv txav, kev daws teeb meem tsis zoo, smearing, los yog distortion ntawm cov protein bands, thiab lwm yam.Peb muab cov ntsiab lus ntau yam teeb meem rau peb cov neeg siv khoom siv kom paub cov laj thawj thiab kom tau txais cov txiaj ntsig raug thiab txhim kho qhov zoo ntawm cov protein sib cais.

Luag ntxhiband- band qauv nkhaus upwards ntawm ob sab ntawm lub gel

Ua rau
1. Qhov nruab nrab ntawm cov gel khiav kub dua li qhov kawg
2. Lub zog muaj zog ntau dhau

Kev daws
① Buffer tsis tov zoo los yog tsis nyob rau hauv lub sab sauv chamber ntau concentrated.Remake tsis, xyuas kom meej sib xyaw, tshwj xeeb tshaj yog thaum diluting 5x lossis 10x Tshuag
② Txo qhov teeb tsa hluav taws xob los ntawm 200 V mus rau 150 V lossis ua kom qis qis rau hauv 1 cm ntawm sab saum toj ntawm phaj luv

Vertical streaking ntawm cov protein

Ua rau
1. Qauv overloaded
2. Kev daws cov nag lossis daus

Kev daws
① Dilute cov qauv, xaiv tshem tawm cov protein ntau hauv cov qauv, lossis txo qhov voltage txog 25% kom txo qis streaking
② Centrifuge qauv ua ntej ntxiv SDS qauv tsis, lossis txo% T ntawm cov gel
③ Qhov piv ntawm SDS rau cov protein yuav tsum txaus los npog txhua cov protein molecule nrog SDS, feem ntau 1.4: 1.Tej zaum nws yuav xav tau SDS ntau dua rau qee cov qauv kuaj cov protein

Bthiabkab rov tavsib kis

Ua rau
1. Diffusion ntawm qhov dej ua ntej tig rau tam sim no
2. Ionic lub zog ntawm cov qauv qis dua li ntawm cov gel

Kev daws
① Txo lub sij hawm nruab nrab ntawm cov qauv thov thiab tig lub zog pib
② Siv tib yam tsis nyob hauv cov qauv xws li hauv cov gel lossis cov gel stacking

Protein bands distorted los yog skewed

Ua rau
1. Tsis zoo polymerization nyob ib ncig ntawm lub qhov dej
2. Cov ntsev hauv cov qauv
3. Tsis sib xws gel interface

Kev daws
① Degas stacking gel tov ua ntej casting;nce ammonium persulfate thiab TEMED concentrations los ntawm 25%, rau stacking gel los yog qis% T, tawm APS tib yam thiab ob npaug rau TEMED concentration.
② Tshem tawm cov ntsev los ntawm kev lim ntshav, tshem tawm;
③ Txo tus nqi polymerization.Overlay gels ua tib zoo.

Beijing Liuyi Biotechnology Company Ltd tsim cov khoom lag luam electrophoresis uas tuaj yeem pab daws cov teeb meem uas peb yuav ntsib hauv peb qhov kev sim electrophoresis.

Beijing Liuyi Biotechnology Company Ltd tau tsim nyob rau xyoo 1970, yav tas los hu ua Beijing Liuyi Instrument Factory, yog lub chaw tsim khoom ntawm cov cuab yeej kuaj thiab cov cuab yeej tshawb fawb raws li hauv Beijing, Suav.Nrog rau ntau tshaj 50 xyoo kev tsim thiab tsim cov khoom rau electrophoresis thiab kev tshawb fawb txog lub neej, nws dhau los ua ib tus neeg muab kev kuaj xyuas cov cuab yeej thiab cov kev daws teeb meem hauv Suav teb.Lub tuam txhab cov khoom lag luam suav nrog ntau yam kev sim ntsuas, suav nrog kab rov tav nucleic acid electrophoresis tank, ntsug protein. electrophoresis tank/unit, black-box type UV analyzer, Gel Document Tracking Imaging Analyzer, thiab electrophoresis fais fab mov.Cov khoom no tau siv dav hauv cov tsev kawm tshawb fawb, tsev kawm qib siab, thiab ntau yam kev lag luam, xws li tshuaj, biotechnology, thiab kev saib xyuas ib puag ncig.Lub tuam txhab yog ISO9001 & ISO13485 certified tuam txhab thiab muaj CE daim ntawv pov thawj.

Muajntau homntsugElectrophoresis Tank rauprotein electrophoresisrau kev tsom xam thiab txheeb xyuas cov qauv ntawm cov protein los ntawm polyacrylamide gel electrophoresis,thiabkuj rau kev ntsuas cov qauv 'qhov hnyav molecular, purifying cov qauv thiab npaj cov qauv.Cov khoom no tau txais tos tag nrho hauvkev lag luam hauv tsev thiab txawv teb chaws.

Lub zog hluav taws xob electrophoresis yog ib qho tseem ceeb ntawm cov khoom sivelectrophoresis system, muab qhov chaw ruaj khov thiab meej ntawm cov hluav taws xob tam sim no los tsav cov txheej txheem sib cais.Itfeem ntau xa cov hluav taws xob tsis tu ncua lossis qhov tam sim no mus rau qhov system electrophoresis, nyob ntawm qhov kev sim tshwj xeeb.Nws kuj tso cai rau tus neeg siv los kho qhov voltage lossis tam sim no cov zis, nrog rau lwm yam tsis xws li lub sijhawm thiab qhov kub thiab txias, txhawm rau txhim kho qhov kev sib cais rau ib qho kev sim tshwj xeeb.

Flos yog saib cov gel, koj tuaj yeem xaiv UV Transilluminator WD-9403 series tsim los ntawm Beijing Liuyi Biotechnology.A UV transilluminator yog lub ntsuas ntsuas siv los ntsuas pom thiab txheeb xyuas DNA, RNA, thiab cov qauv protein.Nws ua haujlwm los ntawm kev teeb tsa cov qauv nrog UV teeb, uas ua rau cov qauv fluoresce thiab pom.Muaj ntau tus qauv ntawm UV transilluminatormuab rau koj los ntawm peb.WD-9403A yog tshwj xeeb rau kev soj ntsuam cov protein electrophoresis, thiab WD-9403F yog siv los soj ntsuam DNA thiab protein electrophoresis.

Cov khoom lag luam no tuaj yeem ua haujlwm los ntawm casting gel mus rau kev soj ntsuam gel raws li koj qhov kev sim xav tau.Qhia rau peb paub koj cov kev xav tau, ob qho tib si OEM, ODM thiab cov neeg xa khoom tau txais tos.We yuav sim peb qhov zoo tshaj plaws los muab koj cov khoom thiab cov kev pabcuam.

Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb.Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.