Polymerase Chain Reaction (PCR) yog cov txheej txheem biology molecular siv los nthuav cov DNA tshwj xeeb. Nws tuaj yeem suav hais tias yog ib daim ntawv tshwj xeeb ntawm DNA replication sab nraum lub cev muaj sia. Lub luag haujlwm tseem ceeb ntawm PCR yog nws lub peev xwm los ua kom muaj txiaj ntsig zoo ntawm DNA.
Txheej txheem cej luam ntawm Polymerase Chain Reaction Cycle
Duab los ntawm Bioninja
Dab tsi yog cov txheej txheem thermal cycling hauv PCR?
PCR muaj peb theem pib:
1. Denaturation ntawm Template DNA: Lub template DNA yog rhuab mus rau ib ncig ntawm 93 ° C rau ib tug tej lub sij hawm, ua rau ob-stranded DNA cais mus rau hauv ib tug strands, npaj nws rau primer binding nyob rau hauv lub tom ntej no cov tshuaj tiv thaiv voj voog.
2. Annealing ntawm Template DNA thiab Primers: Tom qab denaturation, qhov kub thiab txias yog txo mus rau ib ncig ntawm 55 ° C, tso cai rau cov primers khi rau lub complementary sequences ntawm ib leeg-stranded template DNA.
3. Extension ntawm Primers: Ntawm 72 ° C, DNA template-primer complex undergoes extension nrog kev pab los ntawm DNA polymerase (xws li Taq DNA polymerase), siv dNTPs ua substrates los ua ke ib tug tshiab complementary strand raws li tus qauv DNA.
Los ntawm kev rov ua dua qhov denaturation, annealing, thiab txuas ntxiv cov txheej txheem, ntau "semi-conservative replication strands" raug tsim, uas tuaj yeem ua cov qauv rau lub voj voog tom ntej. Txhua lub voj voog yuav siv li 2 mus rau 4 feeb, tso cai rau cov noob caj noob ces kom nthuav dav ntau lab lub sijhawm hauv 2 mus rau 3 teev.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Sep-24-2024