Koj puas muaj teeb meem hauv kev npaj agarose gel?Cia ua raws lipeb cov kws tshaj lij hauv kev npaj cov gel.
Cov txheej txheem kev npaj ntawm agarose gel suav nrog cov kauj ruam hauv qab no:
Ntsuas Agarose Powder
Ntsuas qhov xav tau ntawm agarose hmoov raws li qhov xav tau concentration rau koj qhov kev sim. Feem ntau agarose concentrations nyob ntawm 0.5% mus rau 3%. Cov concentrations siab dua yog siv los cais cov DNA me me molecules, thaum cov concentrations qis dua yog rau cov molecules loj.
Kev npaj Buffer Solution
Ntxiv cov hmoov agarose rau qhov tsim nyog electrophoresis tsis, xws li 1 × TAE lossis 1 × TBE. Qhov ntim ntawm qhov tsis yuav tsum sib phim qhov ntim ntawm gel xav tau rau koj qhov kev sim.
Dissolving lub Agarose
Tshav kub lub agarose thiab tsis sib tov kom txog thaum agarose dissolves tag. Qhov no tuaj yeem ua tiav siv lub microwave lossis lub phaj kub. Do cov tshuaj intermittently los tiv thaiv nws los ntawm boiling. Cov tshuaj agarose yuav tsum ua kom pom tseeb yam tsis muaj qhov pom pom.
Txias cov tshuaj Agarose
Cia cov rhuab agarose tov kom txias rau ib ncig ntawm 50-60 ° C. Do cov tshuaj thaum lub sij hawm txias txheej txheem los tiv thaiv ntxov ntxov solidification.
Ntxiv Nucleic Acid Stain (yeem)
Yog tias koj xav pom DNA lossis RNA hauv cov gel, koj tuaj yeem ntxiv cov nucleic acid stain, xws li GelRed lossis ethidium bromide, nyob rau theem no. Thaum tuav cov stains no, hnav hnab looj tes thiab siv ceev faj, vim tias lawv tuaj yeem ua rau muaj kuab lom.
Casting lub Gel
Ncuav cov kua agarose txias rau hauv ib qho npaj electrophoresis gel pwm. Ntxig ib lub zuag los tsim cov qauv qhov dej, kom ntseeg tau tias cov zuag tau ruaj ntseg thiab cov tshuaj sib npaug sib npaug hauv cov pwm.
Gel Solidification
Cia cov gel kom khov ntawm chav tsev kub, uas feem ntau yuav siv 20-30 feeb nyob ntawm qhov concentration thiab tuab ntawm cov gel.
Rtshem cov Comb
Thaum cov gel tau ruaj khov, ua tib zoo tshem cov zuag kom pom cov qauv qhov dej. Muab cov gel nrog rau cov pwm rau hauv lub tshuab electrophoresis thiab npog nws nrog tus nqi tsim nyog ntawm electrophoresis tsis, kom ntseeg tau tias cov gel yog tag nrho submerged.
Npaj rau Electrophoresis
Tom qab cov gel npaj txhij, thauj koj cov qauv mus rau hauv lub qhov dej, thiab pib nrog kev sim electrophoresis.
Yog tias koj muaj teeb meem nrog kev npaj gel, thov hu rau peb. Peb muaj cov kws tshaj lij lab kws tshaj lij muaj kev sib txuas lus nrog koj.
Peb zoo siab los qhia qee cov xov xwm zoo: peb cov DYCP-31DN kab rov tav electrophoresis tank yog tam sim no ntawm kev nce qib, tiv tauj peb kom paub ntau ntxiv tam sim no!
DYCP-31DN kab rov tav electrophoresis tank
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Oct-15-2024