Comet Assay (Single Cell Gel Electrophoresis, SCGE) yog cov txheej txheem rhiab heev thiab ceev ceev feem ntau siv los txheeb xyuas DNA puas thiab kho hauv ib lub hlwb. Lub npe "Comet Assay" los ntawm cov yam ntxwv zoo li lub hnub qub uas tshwm sim hauv cov txiaj ntsig: lub nucleus ntawm lub xov tooj ntawm tes tsim "lub taub hau," thaum cov DNA puas tawg tawg, tsim "tail" zoo li lub hnub qub.
Txoj Cai
Lub hauv paus ntsiab lus ntawm Comet Assay yog raws li kev tsiv teb tsaws ntawm DNA tawg nyob rau hauv ib qho hluav taws xob teb. Tsis zoo DNA nyob hauv lub cell nucleus, thaum puas los yog tawg DNA migrate mus rau lub anode, tsim lub comet "tail." Qhov ntev thiab siv ntawm tus Tsov tus tw yog ncaj qha proportional rau qhov DNA puas.
Txheej txheem
- Kev npaj ntawm tes: Cells yuav tsum tau kuaj yog tov nrog low-melting-point agarose thiab kis tau rau ntawm lub tshuab nqus tsev slides los tsim ib txheej txheej.
- Cell Lysis: Cov slides yog immersed nyob rau hauv ib tug lysis tov kom tshem tawm cov cell membrane thiab nuclear membrane, exposing DNA.
- Electrophoresis: Cov slides yog muab tso rau hauv ib lub electrophoresis chamber nyob rau hauv alkaline los yog nruab nrab tej yam kev mob. Cov DNA puas tsuaj tsiv mus rau qhov zoo electrode nyob rau hauv lub zog ntawm hluav taws xob teb.
- Kev pleev xim: Tom qab electrophoresis, cov slides yog stained nrog fluorescent dye (xws li, ethidium bromide) kom pom cov DNA.
- Microscopic Analysis: Siv lub fluorescence microscope los yog software tshwj xeeb, cov duab comet raug tshuaj xyuas, thiab ntsuas qhov ntev thiab qhov hnyav.
Duab los ntawm biorender
Kev txheeb xyuas cov ntaub ntawv
Cov txiaj ntsig tau los ntawm Comet Assay raug ntsuas raws li ntau qhov tseem ceeb:
- Tail Ntev: Sawv cev qhov deb ntawm DNA migrate, qhia txog qhov kev puas tsuaj DNA.
- Tail DNA Cov ntsiab lus: Qhov feem pua ntawm DNA uas txav mus rau hauv tus Tsov tus tw, feem ntau siv los ua qhov ntsuas ntau ntawm DNA puas.
- Olive Tail Moment (OTM): Sib txuas ob qhov ntev thiab tus Tsov tus tw DNA cov ntsiab lus los muab kev ntsuas ntau dua ntawm DNA puas.
Daim ntawv thov
- Kev tshawb fawb Genotoxicity: Lub Comet Assay tau dav siv los ntsuas qhov cuam tshuam ntawm cov tshuaj, tshuaj, thiab hluav taws xob ntawm cell DNA, ua rau nws yog ib qho cuab yeej tseem ceeb rau kev kuaj genotoxicity.
- Environmental Toxicology: Nws pab txheeb xyuas qhov cuam tshuam ntawm ib puag ncig cov pa phem ntawm DNA ntawm cov kab mob, muab kev nkag siab txog kev nyab xeeb ntawm ecosystem.
- Kev Tshawb Fawb Txog Kev Kho Mob thiab Kev Kho Mob: Lub Comet Assay yog siv los kawm txog DNA kho mechanisms, mob qog noj ntshav, thiab lwm yam kab mob DNA. Nws kuj tseem ntsuas qhov cuam tshuam ntawm kev kho mob qog noj ntshav xws li kev kho hluav taws xob thiab tshuaj khomob ntawm DNA.
- Food and Agriculture Sciences: Siv los ntsuas kev nyab xeeb ntawm cov tshuaj tua kab, khoom noj khoom haus additives, thiab lwm yam khoom, thiab los kawm txog lawv cov tshuaj lom nyob rau hauv cov qauv tsiaj.
Qhov zoo
- Siab rhiab heev: Muaj peev xwm txheeb xyuas cov qib DNA tsis zoo.
- Kev ua haujlwm yooj yim: Cov txheej txheem yog ncaj nraim, ua rau nws haum rau high-throughput kev tshuaj ntsuam.
- Kev thov dav: Nws tuaj yeem siv rau ntau hom cell, nrog rau cov tsiaj txhu thiab cov nroj tsuag.
- Quantification Challenges: Thaum muab cov ntaub ntawv zoo ntawm DNA puas ntsoog, kev tsom xam ntau nyob ntawm cov software thiab cov duab tsom xam cov tswv yim.
- Kev sim tej yam kev mob: Cov txiaj ntsig tuaj yeem cuam tshuam los ntawm cov yam ntxwv xws li lub sijhawm electrophoresis thiab pH, yuav tsum tau ua tib zoo tswj cov kev sim.
Kev txwv
Lub Comet Assay yog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb biomedical, kev tshawb fawb ib puag ncig, thiab kev txhim kho tshuaj vim nws qhov yooj yim thiab muaj kev nkag siab zoo hauv kev tshawb nrhiav DNA puas thiab kho. Beijing Liuyi Biotechnology Co., Ltd (Liuyi Biotechnology)muaj kab rov tav electrophoresis chamber rau comet assay. Txais tos tiv tauj peb los tham txog covComet Assayraws tu qauv.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Sep-20-2024