Pulsed Field Gel Electrophoresis CHEF Mapper A1
Specifications
| Qauv | CHEF Mapper A1 |
| Voltage gradient | 0.5V / cm rau 9.6V / cm, incremented 0.1V / cm |
| Qhov siab tshaj tam sim no | 0.5 A |
| Qhov siab tshaj plaws voltage | 350v ua |
| Pulse Angle | ± 120 ° |
| Sijhawm Gradient | Tawm |
| Hloov Sijhawm | 50ms rau 18h |
| Lub sijhawm ua haujlwm siab tshaj plaws | 999h ua |
| Tus naj npawb ntawm Electrodes | 24, ywj siab tswj |
| Qhov kub thiab txias | 0 ℃ to 50 ℃, kuaj pom yuam kev < ± 0.5 ℃ |
Kev piav qhia
Pulsed teb gel electrophoresis (PFGE) cais DNA molecules los ntawm alternating lub hluav taws xob teb ntawm txawv spatial oriented electrode khub, ua rau DNA molecules, uas yuav ua tau tsheej lab ntawm lub hauv paus khub ntev mus reorient thiab migrate los ntawm agarose gel pores ntawm txawv speeds.Nws ua tiav cov kev daws teeb meem siab nyob rau hauv qhov ntau thiab feem ntau yog siv hauv cov khoom siv hluavtaws;kev txheeb xyuas cov kab mob lom thiab microbial;kev tshawb fawb hauv molecular epidemiology;kev tshawb fawb ntawm loj plasmid fragments;localization ntawm cov noob kab mob;daim ntawv qhia lub cev ntawm cov noob, RFLP tsom, thiab DNA ntiv tes;programmed cell tuag tshawb nrhiav;kev tshawb fawb txog DNA puas thiab kho;Kev cais tawm thiab kev txheeb xyuas ntawm genomic DNA;kev sib cais ntawm chromosomal DNA;kev tsim kho, kev txheeb xyuas, thiab kev tshuaj xyuas ntawm cov tsev qiv ntawv genomic loj;thiab transgenic research.t concentrations tsawg li 0.5 ng/µL (dsDNA).
Daim ntawv thov
Haum rau kev tshawb nrhiav thiab cais DNA molecules xws li 100bp txog 10Mb hauv qhov loj me, ua tiav qhov kev daws teeb meem siab hauv qhov ntau.
Feature
• Advanced Technology: Muab CHEF thiab PACE pulsed-field technologies kom ua tiav cov txiaj ntsig zoo nrog txoj kab ncaj nraim, tsis khoov.
• Independent Control: Nta 24 ntawm nws tus kheej tswj platinum electrodes (0.5mm txoj kab uas hla), nrog rau txhua tus electrode hloov tau ib tus zuj zus.
• Tsis siv neeg suav muaj nuj nqi: Integrate ntau yam tseem ceeb variables xws li voltage gradient, kub, hloov lub kaum sab xis, pib lub sij hawm, kawg lub sij hawm, tam sim no hloov lub sij hawm, tag nrho cov khiav lub sij hawm, voltage, thiab tam sim no rau kev siv tsis siv neeg xam, pab cov neeg siv kom tiav kev soj ntsuam tej yam kev mob.
• Unique Algorithm: Siv lub cim pulse tswj algorithm rau kev sib cais zoo dua, yooj yim sib txawv ntawm cov kab thiab voj DNA, nrog kev sib cais ntawm cov DNA loj loj.
• Automation: Cia li sau thiab rov pib electrophoresis yog tias lub kaw lus cuam tshuam vim lub zog tsis ua haujlwm.
• Tus neeg siv-Configurable: Tso cai rau cov neeg siv teeb tsa lawv tus kheej.
• Flexibility: Lub kaw lus tuaj yeem xaiv qhov tshwj xeeb voltage gradients thiab hloov sijhawm rau qee qhov DNA loj.
• Loj Screen: Nruab nrog 7-nti LCD npo rau kev ua haujlwm yooj yim, muaj qhov tshwj xeeb software tswj rau kev siv yooj yim thiab yooj yim.
• Kev Ntsuas Kub: Dual kub sojntsuam ncaj qha kuaj tsis tau qhov ntsuas kub nrog qhov ua yuam kev ntawm tsawg dua ± 0.5 ℃.
• Circulation System: Los nrog cov kab mob tsis sib xws uas tswj tau thiab saib xyuas qhov ntsuas kub tsis zoo, ua kom qhov kub thiab txias tsis tu ncua thaum lub sij hawm electrophoresis.
• Kev nyab xeeb siab: suav nrog cov pob tshab acrylic kev nyab xeeb uas tau txiav tawm lub zog thaum nqa, nrog rau kev tiv thaiv ntau dhau thiab tsis muaj kev tiv thaiv.
• Adjustable Leveling: Lub tank electrophoresis thiab gel caster feature adjustable taw rau leveling.
• Pwm Tsim: Lub tank electrophoresis yog tsim los nrog cov qauv pwm sib xyaw tsis muaj kev sib txuas;lub electrode khib yog nruab nrog 0.5 hli platinum electrodes, kom ntseeg tau tias durability thiab ruaj khov kev sim.
FAQ
Q: Dab tsi yog Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis yog cov txheej txheem siv rau kev sib cais ntawm DNA loj molecules raws li lawv qhov loj me.Nws suav nrog kev hloov pauv cov kev taw qhia ntawm cov hluav taws xob hauv hluav taws xob hauv gel matrix kom muaj kev sib cais ntawm DNA tawg uas loj dhau los daws los ntawm cov tshuaj agarose gel electrophoresis.
Q: Dab tsi yog daim ntawv thov ntawm Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis yog dav siv hauv molecular biology thiab noob caj noob ces:
Daim ntawv qhia txog DNA loj molecules, xws li chromosomes thiab plasmids.
• Txiav txim genome loj.
• Kawm txog kev hloov ntawm caj ces thiab kev sib raug zoo evolutionary.
• Molecular epidemiology, tshwj xeeb tshaj yog rau kev taug qab cov kab mob sib kis.
• Kev tshuaj xyuas DNA puas thiab kho.
• Txiav txim seb muaj cov noob caj noob ces lossis DNA ib ntus.
Q: Pulsed Field Gel Electrophoresis ua haujlwm li cas?
A: Pulsed Field Gel Electrophoresis ua haujlwm los ntawm kev xa cov DNA molecules mus rau lub zog hluav taws xob uas hloov pauv hauv kev coj ua.Qhov no tso cai rau DNA loj molecules rau reorient lawv tus kheej ntawm pulses, ua rau lawv txav los ntawm gel matrix.Cov DNA me me txav nrawm dua los ntawm cov gel, thaum cov loj dua txav qeeb dua, tso cai rau lawv sib cais raws li qhov loj me.
Q: Dab tsi yog lub hauv paus ntsiab lus tom qab Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis cais DNA molecules raws li lawv qhov loj los ntawm kev tswj lub sijhawm thiab kev taw qhia ntawm cov hluav taws xob pulses.Kev hloov pauv ua rau DNA loj molecules mus txuas ntxiv rau lawv tus kheej, ua rau lawv tsiv teb tsaws los ntawm gel matrix thiab sib cais raws li qhov loj me.
Q: Dab tsi yog qhov zoo ntawm Pulsed Field Gel Electrophoresis?
A: Kev daws teeb meem siab rau kev sib cais loj DNA molecules txog li ob peb lab lub hauv paus khub. Muaj peev xwm daws thiab paub qhov txawv DNA ntawm qhov ntau thiab tsawg.Versatility nyob rau hauv daim ntawv thov, los ntawm microbial typing mus rau molecular noob caj noob ces thiab genomics.Tsim txoj kev rau epidemiological kev tshawb fawb thiab genetic mapping.
Q: Cov khoom siv dab tsi xav tau rau Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis feem ntau xav tau lub tshuab hluav taws xob electrophoresis nrog cov electrodes tshwj xeeb rau kev tsim cov teb.Agarose gel matrix nrog qhov tsim nyog concentration thiab tsis.Fais fab mov muaj peev xwm ntawm generating high-voltage pulses.Cooling system kom dissipate tshav kub generated thaum lub sij hawm electrophoresis, thiab ib ncig lub twj tso kua mis.
