PCR Thermal Cycler WD-9402M
Specifications
| Qauv | WD-9402M |
| Muaj peev xwm | 96 x 0.2 ml |
| Tube | 96x0.2ml (PCR phaj tsis muaj / semi tiab), 12x8x0.2ml strips, 8x12x0.2ml strips, 0.2ml raj (qhov siab 20 ~ 23mm) |
| Block Temperature Range | 0-105 ℃ |
| Thaiv qhov ntsuas kub qhov tseeb | ± 0.2 ℃ |
| Thaiv qhov kub tsis sib xws | ± 0.5 ℃ |
| Kub nce tus nqi (nruab nrab) | 4 ℃ |
| Cooling down Rate (nruab nrab) | 3 ℃ |
| Kev Tswj Kub Kub | Thaiv/Tube |
| Gradient Temp. Ntau yam | 30-105 ℃ |
| Max.Heating Rate | 5 ℃ / s |
| Max.Cooling Rate 4.5 ℃ / S | 4.5 ℃ / s |
| Gradient Teeb Span | Max. 42 ℃ |
| Gradient Temperature raug | ± 0.3 ℃ |
| Temperature zaub raug | 0.1 ℃ |
| Cua sov Lid Qhov kub thiab txias | 30 ℃ ~ 110 ℃ |
| Tsis siv neeg cua sov lub hau | Kaw txiav tawm thaum tus qauv qis dua 30 ℃ lossis qhov kev pab cuam dhau |
| Timer nce / txo qis | -599 ~ 599 S rau Ntev PCR |
| Kub nce / txo qis | -9.9 ~ 9.9 ℃ rau Touchdown PCR |
| Timer | 1s ~ 59min59sec / Infinite |
| Cov kev zov me nyuam khaws cia | 10000+ |
| Max. Cycles | 99 |
| Max. Cov kauj ruam | 30 |
| Pause Function | Yog lawm |
| Touchdown muaj nuj nqi | Yog lawm |
| Ntev PCR Function | Yog lawm |
| Lus | Lus Askiv |
| Program Pause Function | Yog lawm |
| 16 ℃ Kub tuav muaj nuj nqi | Tsis kawg |
| Lub sijhawm ua haujlwm tiag tiag | Duab-ntawv tso tawm |
| Kev sib txuas lus | USB 2.0 |
| Qhov ntev | 200mm × 300mm × 170mm (W × D × H) |
| Qhov hnyav | 4.5kg ib |
| Fais fab mov | 100-240VAC, 50/60Hz, 600W |
Kev piav qhia
Lub thermal cycler ua haujlwm los ntawm kev rov ua cua sov thiab txias cov tshuaj tiv thaiv sib xyaw uas muaj DNA lossis RNA qauv, primers, thiab nucleotides. Qhov kub thiab txias cycling yog tswj kom ua tiav qhov tsim nyog denaturation, annealing, thiab txuas ntxiv cov kauj ruam ntawm PCR txheej txheem.
Feem ntau, lub thermal cycler muaj qhov thaiv uas muaj ntau lub qhov dej los yog cov raj uas muaj cov tshuaj tiv thaiv sib xyaw, thiab qhov kub ntawm txhua qhov dej yog tswj nws tus kheej. Lub thaiv yog rhuab thiab txias siv lub Peltier caij los yog lwm yam cua sov thiab cua txias system.
Feem ntau cov thermal cyclers muaj cov neeg siv-phooj ywg interface uas tso cai rau tus neeg siv los ua haujlwm thiab kho cov kev caij tsheb kauj vab, xws li qhov kub thiab txias, ncua sij hawm, thiab ntau lub voj voog. Lawv kuj tseem tuaj yeem muaj cov zaub los saib xyuas qhov kev nce qib ntawm cov tshuaj tiv thaiv, thiab qee cov qauv yuav muab cov yam ntxwv siab heev xws li kev tswj qhov kub thiab txias, ntau yam thaiv kev teeb tsa, thiab kev saib xyuas thiab tswj cov chaw taws teeb.
Daim ntawv thov
Polymerase Chain Reaction (PCR) yog cov txheej txheem molecular biology dav siv rau ntau yam kev siv. Qee qhov kev siv PCR feem ntau suav nrog:
DNA Amplification: Lub hom phiaj tseem ceeb ntawm PCR yog kom nthuav dav DNA ib ntus. Qhov no yog qhov tseem ceeb kom tau txais cov DNA txaus rau kev soj ntsuam ntxiv lossis kev sim.
Kev kuaj caj ces: PCR yog siv dav hauv kev kuaj caj ces txhawm rau txheeb xyuas cov cim caj ces lossis kev hloov pauv cuam tshuam nrog cov kab mob. Nws yog ib qho tseem ceeb rau kev kuaj mob thiab kawm txog cov kab mob genetic predispositions.
DNA Cloning: PCR yog ua hauj lwm los tsim kom muaj ntau ntawm cov DNA tshwj xeeb, uas tuaj yeem raug cloned rau hauv vector rau kev tswj xyuas ntxiv lossis kev tshuaj xyuas.
Kev Tshawb Fawb DNA Kev Tshawb Fawb: PCR yog qhov tseem ceeb hauv kev tshawb fawb forensic rau kev nthuav dav cov qauv DNA tau txais los ntawm kev ua txhaum cai. Nws pab txheeb xyuas tib neeg thiab tsim kev sib raug zoo ntawm caj ces.
Microbial Detection: PCR yog siv los kuaj xyuas cov kab mob microbial hauv kev kuaj mob lossis kuaj ib puag ncig. Nws tso cai rau kev txheeb xyuas cov kab mob sai sai.
Quantitative PCR (qPCR lossis Real-Time PCR): qPCR tso cai rau kom muaj nuj nqis ntawm DNA thaum lub sijhawm ua kom nthuav dav. Nws yog siv los ntsuas cov theem ntawm cov noob caj noob ces, kuaj xyuas cov kab mob kis tau, thiab ntsuas tus lej ntawm cov DNA tshwj xeeb.
Molecular Evolution Studies: PCR yog siv rau hauv kev tshawb fawb soj ntsuam kev hloov pauv caj ces hauv cov neeg, kev sib raug zoo ntawm kev hloov pauv, thiab kev tshuaj ntsuam phylogenetic.
Environmental DNA (eDNA) Kev Ntsuam Xyuas: PCR yog ua haujlwm los kuaj pom cov kab mob tshwj xeeb hauv cov qauv ib puag ncig, pab txhawb rau kev tshawb fawb txog biodiversity thiab ecological.
Genetic Engineering: PCR yog lub cuab yeej tseem ceeb hauv kev tsim cov noob caj noob ces los qhia txog DNA ib ntus rau hauv cov kab mob. Nws yog siv los tsim cov kab mob hloov pauv caj ces (GMOs).
Sequencing Library Preparation: PCR koom nrog hauv kev npaj cov tsev qiv ntawv DNA rau tiam tom ntej sequencing technologies. Nws pab ua kom cov DNA tawg rau hauv cov ntawv sau cia hauv qab.
Site-Directed Mutagenesis: PCR yog siv los qhia txog kev hloov pauv hauv DNA ib ntus, tso cai rau cov kws tshawb fawb los kawm txog qhov cuam tshuam ntawm kev hloov caj ces tshwj xeeb.
DNA Fingerprinting: PCR yog siv rau hauv cov txheej txheem DNA ntiv tes rau kev txheeb xyuas tus kheej, kuaj tus txiv, thiab tsim kev sib raug zoo lom neeg.
Feature
•Elegant tsos, compact loj, thiab nruj qauv.
•Tshwj xeeb nrog kev ua haujlwm siab, ntsiag to axial-flow ntxuam rau kev ua haujlwm ntsiag to.
•Features dav gradient muaj nuj nqi ntawm 30 ℃, cia optimization ntawm kev sim tej yam kev mob kom tau raws li cov kev sim nruj heev.
• 5-nti high-txhais xim touchscreen rau kev ua haujlwm yooj yim thiab yooj yim, ua kom yooj yim kho, txuag, thiab khiav ntawm cov haujlwm.
•Industrial-qib kev ua haujlwm, pab txhawb kev ua haujlwm tsis tu ncua thiab tsis ua yuam kev 7x24.
• Cov ntaub ntawv ceev ceev hloov mus rau USB flash drive rau kev pab cuam yooj yim thaub qab, txhim kho cov ntaub ntawv khaws cia.
•Advanced semiconductor txias tshuab thiab tshwj xeeb PID kub tswj tshuab txhawb nqa tag nrho cov kev ua tau zoo rau qhov siab tshiab: siab kub tswj qhov tseeb, ceev cua sov thiab cua txias, thiab uniformly faib module kub.
FAQ
Q: Dab tsi yog thermal cycler?
A: Lub thermal cycler yog cov cuab yeej siv hauv chav kuaj siv los nthuav qhia DNA lossis RNA sib lawv liag los ntawm polymerase saw cov tshuaj tiv thaiv (PCR). Nws ua haujlwm los ntawm kev caij tsheb kauj vab los ntawm kev hloov pauv ntawm qhov kub thiab txias, tso cai rau DNA ib ntus kom nthuav dav.
Q: Dab tsi yog cov khoom tseem ceeb ntawm lub thermal cycler?
A: Cov khoom tseem ceeb ntawm lub tshuab hluav taws xob muaj xws li lub tshuab cua sov, lub tshuab hluav taws xob, lub tshuab hluav taws xob, qhov ntsuas kub, lub tshuab hluav taws xob, thiab lub vaj huam sib luag.
Q: Lub thermal cycler ua haujlwm li cas?
A: Lub thermal cycler ua haujlwm los ntawm cua sov thiab txias DNA qauv nyob rau hauv ib tug series ntawm kub mus. Cov txheej txheem kev caij tsheb kauj vab suav nrog denaturation, annealing, thiab ncua sij hawm, txhua qhov kub thiab sijhawm. Cov voj voog no tso cai rau qee qhov DNA ua ntu zus los ntawm cov tshuaj tiv thaiv polymerase saw (PCR).
Q: Dab tsi yog cov yam ntxwv tseem ceeb uas yuav tsum tau xav txog thaum xaiv lub thermal cycler?
A: Qee qhov tseem ceeb uas yuav tsum tau xav txog thaum xaiv lub thermal cycler suav nrog tus naj npawb ntawm cov qhov dej lossis cov tshuaj tiv thaiv, qhov kub thiab txias thiab qhov ceev ceev, qhov tseeb thiab qhov sib xws ntawm kev tswj qhov kub thiab txias, thiab tus neeg siv interface thiab software muaj peev xwm.
Q: Koj ua li cas tswj lub thermal cycler?
A: Yuav kom tswj tau lub thermal cycler, nws yog ib qho tseem ceeb kom tsis tu ncua ntxuav cov cua sov thaiv thiab cov hlab ntsha, xyuas qhov hnav thiab rhuav ntawm cov khoom, thiab ntsuas qhov ntsuas kub kom ntseeg tau tias qhov ntsuas kub zoo thiab zoo ib yam. Nws tseem yog ib qho tseem ceeb uas yuav tau ua raws li cov chaw tsim khoom cov lus qhia rau kev tu thiab kho txhua hnub.
Q: Dab tsi yog qee qhov teeb meem daws teeb meem rau lub thermal cycler?
A: Qee cov txheej txheem daws teeb meem rau lub thermal cycler suav nrog kev kuaj xyuas cov khoom xoob lossis puas, txheeb xyuas qhov ntsuas kub thiab sijhawm, thiab kuaj cov tshuaj tiv thaiv cov raj lossis cov phaj rau kev sib kis lossis kev puas tsuaj. Nws tseem yog ib qho tseem ceeb kom xa mus rau cov chaw tsim khoom cov lus qhia rau kev daws teeb meem tshwj xeeb thiab cov kev daws teeb meem.
