Polyacrylamide gel electrophoresis yog dab tsi?

Polyacrylamide Gel Electrophoresis

Gel electrophoresis yog cov txheej txheem tseem ceeb hauv chav kuaj thoob plaws cov kev qhuab qhia lom neeg, tso cai rau kev sib cais ntawm macromolecules xws li DNA, RNA thiab cov proteins. Cov xov xwm sib cais sib txawv thiab cov txheej txheem tso cai rau cov txheej txheem ntawm cov molecules sib cais kom zoo dua los ntawm kev siv lawv cov yam ntxwv ntawm lub cev. Rau cov protein tshwj xeeb, polyacrylamide gel electrophoresis (PAGE) feem ntau yog cov txheej txheem ntawm kev xaiv.

PAGE yog ib qho txheej txheem uas cais cov macromolecules xws li cov proteins raws li lawv cov electrophoretic txav mus los, uas yog, lub peev xwm ntawm cov analytes txav mus rau ib qho electrode ntawm tus nqi rov qab. Hauv PAGE, qhov no yog txiav txim los ntawm tus nqi, qhov loj me (qhov hnyav molecular) thiab cov duab ntawm cov molecule. Analytes txav los ntawm pores tsim nyob rau hauv polyacrylamide gel. Tsis zoo li DNA thiab RNA, cov proteins sib txawv raws li cov amino acids koom nrog, uas tuaj yeem cuam tshuam lawv txoj haujlwm. Amino acid cov hlua kuj tseem tuaj yeem tsim cov qauv theem nrab uas cuam tshuam rau lawv qhov loj me thiab yog li lawv tuaj yeem txav mus los ntawm qhov hws. Tej zaum nws yuav yog li qee zaum xav tau denature proteins ua ntej electrophoresis rau linearize lawv yog tias xav tau kev kwv yees ntau dua ntawm qhov loj.

SDS PAGE

Sodium-dodecyl sulfate polyacrylamide gel electrophoresis yog txheej txheem siv los cais cov protein molecules ntawm pawg 5 txog 250 kDa. Cov proteins yog cais los ntawm lawv qhov hnyav molecular. Sodium dodecyl sulfate, ib qho anionic surfactant, yog ntxiv rau hauv kev npaj cov gels uas npog cov nqi ntawm cov qauv ntawm cov protein thiab muab cov nqi zoo sib xws rau qhov sib piv loj. Hauv cov lus yooj yooj yim, nws denatures cov proteins thiab muab lawv tus nqi tsis zoo.

Native PAGE

Native PAGE yog cov txheej txheem uas siv cov gels uas tsis yog-denatured rau kev sib cais ntawm cov protein. Tsis zoo li SDS PAGE, tsis muaj tus neeg sawv cev denaturing ntxiv rau hauv kev npaj cov gels. Raws li qhov tshwm sim, kev sib cais ntawm cov protein yuav tshwm sim los ntawm kev them nqi thiab qhov loj ntawm cov proteins. Nyob rau hauv cov txheej txheem no, conformation, folding thiab amino acid chains ntawm cov proteins yog qhov tseem ceeb uas kev sib cais yog nyob ntawm seb. Cov proteins tsis puas hauv cov txheej txheem no, thiab tuaj yeem rov qab tau tom qab ua tiav ntawm kev sib cais.

Polyacrylamide gel electrophoresis (PAGE) ua haujlwm li cas?

Lub hauv paus ntsiab lus ntawm PAGE yog cais cov analytes los ntawm kev hla lawv los ntawm cov pores ntawm polyacrylamide gel siv cov hluav taws xob tam sim no. Txhawm rau ua tiav qhov no, kev sib xyaw acrylamide-bisacrylamide yog polymerized (polyacrylamide) los ntawm kev ntxiv ntawm ammonium persulfate (APS). Cov tshuaj tiv thaiv, uas yog catalyzed los ntawm tetramethylethylenediamine (TEMED), tsim ib tug zoo li cov qauv nrog pores los ntawm cov analytes yuav txav tau (Daim duab 2). Qhov siab dua qhov feem pua ​​​​ntawm tag nrho cov acrylamide suav nrog hauv cov gel, qhov me me ntawm qhov pore, yog li cov proteins me me uas yuav dhau mus. Qhov piv ntawm acrylamide rau bisacrylamide kuj yuav cuam tshuam qhov pore loj tab sis qhov no feem ntau khaws cia tas li. Cov pore me me kuj txo qhov ceev ntawm cov protein me me tuaj yeem txav mus los ntawm cov gel, txhim kho lawv cov kev daws teeb meem thiab tiv thaiv lawv los ntawm kev khiav tawm mus rau hauv qhov tsis nrawm thaum siv tam sim no.

Khoom siv rau Polyacrylamide Gel Electrophoresis

Gel Electrophoresis Cell (Tank / Chamber)
Lub gel tank rau polyacrylamide gel electrophoresis (PAGE) yog txawv ntawm agarose gel tank. Lub agarose gel tank yog kab rov tav, thaum PAGE tank yog ntsug. Los ntawm ntsug electrophoresis cell (tank / chamber), ib tug nyias gel (feem ntau 1.0mm los yog 1.5mm) yog poured ntawm ob iav daim hlau thiab mounted kom lub qab ntawm cov gel yog submerged nyob rau hauv tsis nyob rau hauv ib chamber thiab sab saum toj yog submerged nyob rau hauv tsis. hauv lwm chav. Thaum siv tam sim no, ib qho me me ntawm qhov tsis tuaj yeem txav mus los ntawm cov gel los ntawm lub chamber sab saum toj mus rau hauv qab chamber. Nrog muaj zog clamps los lav lub rooj sib dhos nyob rau hauv txoj hauj lwm upright, cov cuab yeej yooj yim ceev ceev gel khiav nrog txawm txias ua rau sib txawv bands.

Beijing Liuyi Biotechnology Co., Ltd. (Liuyi Biotechnology) manufactures ntau qhov ntau thiab tsawg ntawm polyacrylamide gel electrophoresis hlwb (tanks/chambers). Cov qauv DYCZ-20C thiab DYCZ-20G yog ntsug electrophoresis hlwb (tanks/chambers) rau DNA sequencing tsom xam. Qee qhov ntsug electrophoresis hlwb (tank / chambers) yog sib xws nrog blotting system, xws li cov qauv DYCZ-24DN, DYCZ-25D thiab DYCZ-25E yog sib xws nrog Western Blotting system qauv DYCZ-40D, DYCZ-40G thiab DYCZ-40F, uas yog siv rau kev hloov cov protein molecule los ntawm gel mus rau daim nyias nyias. Tom qab SDS-PAGE electrophoresis, Western Blotting yog cov txheej txheem los kuaj xyuas cov protein tshwj xeeb hauv cov protein sib xyaw. Koj tuaj yeem xaiv cov tshuab blotting no raws li kev sim xav tau.

Electrophoresis Power Supply
Txhawm rau muab hluav taws xob rau kev khiav lub gel, koj yuav xav tau lub zog hluav taws xob electrophoresis. Ntawm Liuyi Biotechnology peb muab ntau yam khoom siv hluav taws xob electrophoresis rau txhua daim ntawv thov. Tus qauv DYY-12 thiab DYY-12C nrog lub zog ruaj khov thiab tam sim no tuaj yeem ua tau raws li qhov yuav tsum tau muaj hluav taws xob siab electrophoresis. Nws muaj kev ua haujlwm ntawm kev sawv ntsug, sijhawm, VH thiab daim ntawv thov ib ntus. Lawv zoo tagnrho rau IEF thiab DNA sequencing electrophoresis daim ntawv thov. Rau daim ntawv thov dav dav ntawm cov protein thiab DNA electrophoresis, peb muaj cov qauv DYY-2C, DYY-6C, DYY-10, thiab lwm yam, uas kuj yog cov khoom siv hluav taws xob kub muag nrog cov khoom siv hluav taws xob electrophoresis (tanks / chambers). Cov no tuaj yeem siv rau hauv nruab nrab thiab tsis tshua muaj hluav taws xob electrophoresis daim ntawv thov, xws li siv rau hauv tsev kawm ntawv lab siv, tsev kho mob lab thiab lwm yam. Ntau cov qauv rau cov khoom siv fais fab, thov mus saib peb lub vev xaib.

Liuyi hom muaj ntau tshaj li 50 xyoo keeb kwm hauv Suav teb thiab lub tuam txhab tuaj yeem muab cov khoom lag luam ruaj khov thiab zoo thoob plaws ntiaj teb. Los ntawm kev txhim kho xyoo, nws tsim nyog rau koj xaiv!

Yog xav paub ntxiv txog peb, thov hu rau peb ntawm email[email tiv thaiv] or [email tiv thaiv].

Cov ntaub ntawv rau Polyacrylamide gel electrophoresis yog dab tsi?
1. Karen Steward PhD Polyacrylamide gel electrophoresis, Nws ua haujlwm li cas, Cov txheej txheem sib txawv, thiab nws cov ntawv thov