Npaj Agarose Gel Rau Electrophoresis

Kev npaj ntawm Agarose Gel rau Electrophoresis

Nco tseg: Yuav tsum hnav cov hnab looj tes pov tseg!

Kauj ruam-by-step cov lus qhia

Ntsuas Agarose Powder:use hnyav daim ntawv thiab ib qho hluav taws xob sib npaug los ntsuas 0.3g ntawm agarose hmoov (raws li 30ml system).

Npaj TBST Buffer:prov kho 30ml ntawm 1x TBST tsis nyob rau hauv 100ml Erlenmeyer hwj.

Dissolving Agarose Hmoov:ppeb cov hmoov agarose rau hauv TBST tsis thiab co zoo.Muab lawv tso rau hauvmus rau lub microwave thiab cua sov (feem ntau rau 50 vib nas this, npog nrog txhuas ntawv ci) kom txog thaum yaj tag.

Txias thiab Ntxiv Nuclease:use hnab looj tes kom tshem tawm cov sib tov los ntawm microwave thiab cia nws txias me ntsis hauv dej txias kom txog thaum nws sov (kwv yees li 60 ° C). Ntxiv 2µl ntawm nuclease (Eb hloov) thaum co kom sib tov kom huv si.

Npaj cov gel pwm:

• Clean thiab qhuav lubgel tais thiab gel casting ntaus ntawvntawm lub tank electrophoresis.
•  Place lub geltaisrau hauv lub tank sab hauv thiab ntxig lub zuag ntawm ib qho chaw ruaj khov.
• Sib tov cov tshuaj agarose gel, txias rau li 65 ° C, thiab ua tib zoo ncuav nws mus rau hauvgel taishauvgel casting khoom, kis nws maj mam kom txog thaum nws tsim ib qho gel txheej ntawm lub khob phaj.
• Cia nws zaum ntawm chav tsev kub kom txog thaum lub gel solidifies tag nrho.
• Maj mam tshem lub zuag vertically thiab tshem tawm daim kab xev.
• Muab lub geltaisnyob rau hauv lub tank electrophoresis.

Tseem ceeb: xyuas kom tsis muaj cua npuas ntawm qhov chaw txhuam hniav. Qhov saum npoo ntawm cov kua yuav tsum du yam tsis muaj ripples.

Khiav lub Gel

Chaw thau khoom Gel

Tom qab cov gel solidifies, muab tso rau hauv lub tank electrophoresis thiab ncuav lub electrophoresis tsis mus txog rau thaum lub gel yog submerged.

Npaj Cov Qauv

• Nqa tus cim thiab thauj khoom tsis pub dhau lub tub yees.
• Ntxiv 6µl ntawm kev thauj khoom tsis rau cov qauv thiab sib tov zoo.
• Siv lub micropipette, maj mam thauj cov qauv mus rau hauv qhov dej loj ntawm cov gel (yuav tsum tsis txhob muab cov gel thiab tsis txhob muab tag nrho cov ntim kom tsis txhob muaj cua npuas).
• Thauj lub cim rau hauv ib qho ntawm cov qhov dej me me (nco ntsoov nws txoj hauj lwm).

Pib Electrophoresis

• Npog lub tank electrophoresis thiab pib electrophoresis tam sim ntawd tom qab thauj cov gel.
• Teem lub voltage rau 60-100V. Cov qauv yuav tsiv los ntawm qhov tsis zoo electrode (dub) mus rau qhov zoo electrode (liab).
• Cov hluav taws xob siab dua ua rau qhov kev sib cais zoo ntawm agarose gel.
• Nres electrophoresis thaum bromophenol xiav dye ncav cuag li 1cm ntawm ntug hauv qab ntawm cov gel phaj.

Kev soj ntsuam cov txiaj ntsig

Tom qab sib cais cov bands, nres electrophoresis, tshem tawm cov gel, thiab ncaj qha ntes thiab yees duab nws.

Siv lub gel imaging system los yees duab thiab saib xyuas cov qhab (xyuas seb puas muaj cov qhab nia rau cov cim lossis cov qauv).

Tom qab tau txais koj daim ntawv qhia gel band, nrhiav tus cim. Raws li tus cim, koj tuaj yeem txiav txim siab lub hom phiaj bands!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tsim cov khoom siv electrophoresis rau ntau tshaj 50 xyoo. Peb nthuav qhia peb kab rov tav electrophoresis tshuab ntawm no rauagarose gel uaelectrophoresis.Xaivkab rov tav electrophoresis systemslos ntawm Beijing liuyi rau koj qhov kev sim gel electrophoresis los pab koj tshawb fawb hauv kev lag luam biotechnology.

Peb muab cov qauv sib txawv ntawm kab rov tav electrophoresis tso tsheb hlau luam rau casting thiab khiav me meagarrosegels lojagarrosecov gels

Xaiv Phau Ntawv Qhia rau Kab rov tav Immersed Gel Electrophoresis Unit

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.

Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.

Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.