Nyob rau hauv ib txwm-evolving field of molecular biology, Polymerase Chain Reaction (PCR) thiab Gel Electrophoresis tau tshwm sim los ua cov txheej txheem pob zeb uas pab txhawb txoj kev kawm thiab kev tswj hwm ntawm DNA. Cov txheej txheem no tsis yog ib qho tseem ceeb rau kev tshawb fawb tab sis kuj muaj kev siv dav hauv kev kuaj mob, kev tshawb fawb forensic, thiab biotechnology.
Duab los ntawm lub vev xaib biology4alevel
PCR yog cov txheej txheem kev hloov pauv uas tsim los ntawm Kary Mullis hauv xyoo 1983, uas tso cai rau cov kws tshawb fawb los nthuav qhia txog DNA ntu exponentially. Cov txheej txheem pib nrog lub denaturation ntawm DNA, qhov twg ob-stranded DNA yog rhuab mus rau ib ncig ntawm 94 ° C, ua rau nws cais mus rau hauv ob lub strands. Qhov no yog ua raws li annealing, qhov twg primers - luv luv sequences ntawm nucleotides - khi rau complementary sequences ntawm ib leeg-stranded DNA ntawm qhov kub thiab txias (feem ntau nyob ib ncig ntawm 55 ° C). Thaum kawg, qhov txuas ntxiv tshwm sim ntawm 72 ° C, qhov twg cov enzyme DNA polymerase ua ke ib txoj hlua tshiab ntawm DNA los ntawm kev ntxiv nucleotides rau cov primers. Lub voj voog no rov ua dua 20-40 zaug, ua rau ntau lab luam ntawm lub hom phiaj DNA ib ntus.
Thaum DNA tau nthuav dav, Gel Electrophoresis tau ua haujlwm los cais thiab txheeb xyuas cov khoom PCR. Cov txheej txheem no suav nrog kev tsiv teb tsaws ntawm DNA tawg los ntawm agarose gel matrix nyob rau hauv kev cuam tshuam ntawm hluav taws xob teb. DNA molecules raug them tsis zoo vim lawv cov pob txha phosphate, thiab lawv tsiv mus rau qhov zoo electrode. Cov gel ua raws li lub sieve, tso cai rau cov DNA me me kom txav nrawm dua li cov loj dua. Raws li qhov tshwm sim, DNA tawg tau raug cais raws li lawv qhov loj me, nrog cov kab sib txawv pom nyob rau hauv lub teeb ultraviolet tom qab staining nrog cov xim zoo li ethidium bromide.
Beijing LIUYIGel Electrophoresis khoom
Kev sib xyaw ntawm PCR thiab Gel Electrophoresis muaj zog hauv ntau daim ntawv thov. Hauv kev kuaj mob, PCR yog siv los kuaj pom muaj cov kab mob, kev hloov pauv caj ces, lossis cov DNA tshwj xeeb uas cuam tshuam txog kab mob. Gel Electrophoresis tom qab ntawd tso cai rau kev pom thiab kev pom zoo ntawm cov kab ke DNA txuas ntxiv. Hauv kev tshawb fawb forensic, cov tswv yim no yog qhov tseem ceeb rau DNA ntiv tes, qhov uas lawv pab kom phim DNA cov qauv los ntawm kev ua txhaum cai nrog cov neeg raug liam.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Beijing LIUYIGel Electrophoresis khoom
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Aug-15-2024