Taw qhia
Gel electrophoresis yog cov txheej txheem tseem ceeb hauv molecular biology, dav siv rau kev sib cais ntawm cov protein, nucleic acids, thiab lwm yam macromolecules. Kev tswj kom zoo ntawm cov qauv ntim, qhov hluav taws xob, thiab lub sijhawm electrophoresis yog qhov tseem ceeb rau kev ua tiav cov txiaj ntsig raug thiab rov tsim dua.Peb cov npoj yaig lab muabCov kev coj ua zoo tshaj plaws rau kev tswj cov kev ntsuas no thaum lub sij hawm SDS-PAGE gel electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis khoom
Qauv Volume: Ua kom muaj kev sib haum xeeb
Thaum ua SDS-PAGE electrophoresis, cov qauv ntim yog qhov tseem ceeb uas tuaj yeem cuam tshuam qhov kev daws teeb meem ntawm koj cov txiaj ntsig. Nws feem ntau pom zoo kom thauj 10 µL ntawm tag nrho cov protein ib qhov dej. Txhawm rau kom muaj kev sib luag thiab tiv thaiv cov qauv sib txawv ntawm cov qhov dej uas nyob ib sab, nws yog ib qho tseem ceeb uas yuav tsum muaj qhov sib npaug ntawm 1x loading buffer hauv txhua qhov dej khoob. Qhov kev ceev faj no yuav pab tiv thaiv kev sib kis ntawm cov qauv mus rau hauv cov kab nyob sib ze, uas tuaj yeem tshwm sim yog tias lub qhov dej tso tseg.
Ua ntej thauj koj cov qauv, ib txwm pib los ntawm kev ntxiv qhov ntsuas qhov hnyav rau ib qhov dej. Qhov no tso cai rau kom yooj yim txheeb xyuas qhov ntau thiab tsawg ntawm cov protein tom qab electrophoresis.
Voltage Control: Ntsuas qhov ceev thiab kev daws teeb meem
Qhov hluav taws xob siv thaum lub sij hawm electrophoresis ncaj qha cuam tshuam rau ob qho tib si ceev ntawm cov qauv tsiv teb tsaws los ntawm cov gel thiab kev daws teeb meem ntawm kev sib cais. Rau SDS-PAGE, nws raug nquahu kom pib nrog qhov tsis tshua muaj hluav taws xob ntawm ib puag ncig 80V. Qhov pib qis qis no tso cai rau cov qauv txav mus qeeb thiab sib npaug, tsom mus rau hauv cov qhab ntse ntse thaum lawv nkag mus rau qhov sib cais gel.
Thaum cov qauv tau nkag mus rau hauv qhov sib cais gel, qhov voltage tuaj yeem nce mus rau 120V. Qhov siab dua qhov hluav taws xob no ua rau kev txav mus los, kom ntseeg tau tias cov proteins tau zoo sib cais raws li lawv qhov hnyav molecular. Nws yog ib qho tseem ceeb los saib xyuas qhov kev nce qib ntawm bromophenol xiav dye pem hauv ntej, uas qhia txog qhov ua tiav ntawm electrophoresis. Rau gels nrog ib tug concentration ntawm 10-12%, 80-90 feeb yog feem ntau txaus; Txawm li cas los xij, rau 15% gels, koj yuav tsum tau ncua sij hawm khiav me ntsis.
Lub Sijhawm Tswjhwm: Paub Thaum Twg
Lub sijhawm yog lwm qhov tseem ceeb hauv gel electrophoresis. Kev khiav lub gel ntev dhau los lossis luv dhau lub sijhawm tuaj yeem ua rau muaj kev sib cais zoo tshaj plaws. Kev tsiv teb tsaws ntawm bromophenol xiav dye yog qhov qhia tau zoo: thaum nws nce mus txog hauv qab ntawm cov gel, feem ntau nws yog lub sijhawm kom tsis txhob khiav. Rau cov qauv gels, xws li 10-12%, electrophoresis ntev li ntawm 80-90 feeb yog feem ntau txaus. Rau cov feem pua siab dua gels, zoo li 15%, lub sijhawm khiav yuav tsum tau txuas ntxiv kom paub meej tias kev sib cais ntawm cov proteins.
Buffer Management: Rov siv dua thiab npaj Buffers
Electrophoresis tsis tuaj yeem rov siv tau 1-2 zaug, nyob ntawm koj qhov chaw kuaj mob tshwj xeeb. Txawm li cas los xij, kom tau txais txiaj ntsig zoo, nws raug nquahu kom npaj cov tshiab 10x tsis thiab dilute nws ua ntej siv. Qhov no ua kom ntseeg tau tias qhov tsis muaj peev xwm tswj tau nws cov txiaj ntsig, ua rau muaj txiaj ntsig zoo dua electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis khoom
Los ntawm kev ua tib zoo tswj cov qauv ntim, qhov hluav taws xob, thiab lub sijhawm electrophoresis, koj tuaj yeem txhim kho qhov raug thiab rov tsim tawm ntawm koj cov txiaj ntsig gel electrophoresis. Kev ua raws li cov kev coj ua zoo tshaj plaws hauv koj qhov chaw ua haujlwm yuav pab koj ua tiav cov qhab nia sib txawv thiab ntau dua, ua rau cov ntaub ntawv zoo dua rau kev txheeb xyuas hauv qab.
Yog tias koj muaj ntau txoj hauv kev zoo los txhim kho gel electrophoresis sim, txais tos los tham nrog peb!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Aug-20-2024