Thaum ua qhov kev sib piv ntawm cov txiaj ntsig electrophoresis, ntau yam yuav ua rau muaj qhov sib txawv ntawm cov ntaub ntawv:
Kev npaj ua qauv:Kev hloov pauv hauv cov qauv concentration, purity, thiab degradation tuaj yeem cuam tshuam cov txiaj ntsig electrophoresis. Cov impurities lossis degraded DNA / RNA nyob rau hauv cov qauv tuaj yeem ua rau smearing lossis tsis tshwj xeeb bands.
Gel Concentration thiab Hom:Cov concentration thiab hom gel (piv txwv li, agarose lossis polyacrylamide) cuam tshuam qhov kev daws teeb meem ntawm molecular sib cais. Cov gels siab dua yog qhov zoo dua rau kev sib cais me me me, thaum qis concentration gels yog tsim rau cov molecules loj.
Electrophoresis tej yam kev mob:Lub zog ntawm cov hluav taws xob hluav taws xob (voltage), lub sijhawm electrophoresis, thiab hom thiab pH ntawm kev khiav tsis tuaj yeem cuam tshuam tag nrho cov txiaj ntsig. Ib qho hluav taws xob ntau dhau tuaj yeem ua rau cov hlua khi lossis txo cov kev daws teeb meem, thiab lub sij hawm txuas ntxiv ntawm electrophoresis tuaj yeem ua rau muaj qhov sib txawv.
Kev ua tau zoo thiab kev npaj ntawm Buffer:Cov buffers tsis raug lossis tas sij hawm tuaj yeem ua rau muaj kev hloov pauv hauv pH thiab ionic zog, cuam tshuam rau kev txav mus los thiab kev daws teeb meem.
Sample Loading npaum li cas thiab cov txheej txheem:Overloading lossis underloading cov qauv tuaj yeem cuam tshuam rau band clarity thiab siv. Kev thauj khoom tsis sib xws tuaj yeem ua rau cov qauv diffusion lossis txoj kab nkhaus.
Electrophoresis Equipment thiab Environmental Conditions: Cov khoom siv electrophoresis sib txawv (xws li gel tso tsheb hlau luam thiab cov khoom siv hluav taws xob) thiab ib puag ncig (xws li kub thiab av noo) tuaj yeem cuam tshuam rau kev ruaj ntseg thiab rov tsim dua ntawm electrophoresis.
Staining thiab Detection Methods:Kev xaiv ntawm stain (xws li, ethidium bromide, SYBR Green) thiab lub sij hawm staining tuaj yeem cuam tshuam rau qhov pom tseeb thiab pom ntawm bands.
Zoo ntawm Electrophoresis Gel:Cov npuas hauv cov gels hauv tsev, cov gels tsis zoo, lossis cov gels degraded tuaj yeem ua rau cov hlua khi los yog tsiv mus nyob txawv txav.
Qauv thiab Loj ntawm DNA / RNA:Txawm hais tias DNA lossis RNA hauv cov qauv yog linear, ncig, lossis supercoiled, lossis qhov loj ntawm cov khoom tawg, yuav cuam tshuam rau lawv cov kev tsiv teb tsaws ceev hauv cov gel.
Sample Handling History:Cov yam ntxwv xws li cov voj voog khov-thaw, qhov kub thiab txias, thiab lub sijhawm tuaj yeem cuam tshuam cov qauv kev ncaj ncees, yog li cuam tshuam rau cov txiaj ntsig electrophoresis.
Liuyi Biotechnology kws tshaj lij ua kev sim electrophoresis ntawm lab
Zoo siab txais tostiv tauj peb los tham txog cov xwm txheej uas yuav ua rau muaj qhov sib txawv ntawm cov ntaub ntawv electrophoresis. Los ntawm kev nkag siab txog cov xwm txheej no, peb tuaj yeem txo qis qhov sib txawv ntawm cov ntaub ntawv, txhim kho qhov rov ua dua tshiab ntawm kev sim thiab qhov tseeb ntawm cov txiaj ntsig..
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam. Peb kuj muab cov cuab yeej kuaj xws li PCR ntsuas, vortex tov khoom thiab centrifuge rau kev kuaj.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Sep-12-2024