Yuav Ua Li Cas Ua DNA Electrophoresis hauv Agarose Gel?

Ntawm no peb yuav piav qhia yuav ua li cas ua agarose gel electrophoresis los ntawm peb tus kws tshawb fawb hauv Liuyi Biotech's lab.

Ua ntej qhov kev sim, peb yuav tsum xyuas cov cuab yeej, cov tshuaj reagents, thiab lwm yam khoom siv thiab cov cuab yeej peb xav tau.

Kev npaj ntawm kev sim apparatuses thiab cov ntaub ntawv

Cov apparatuses rau agarose gel electrophoresis

Kab rov tav electrophoresis cell (tank / chamber), nruab nrab thiab qis voltage electrophoresis fais fab mov, gel duab & tsom xam system.

Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) muaj cov qauv sib txawv ntawm kab rov tav electrophoresis cell (tank / chamber) thiab cov khoom siv hluav taws xob nrog rau cov ntaub ntawv gel. Tus qauv DYCP-31 series khoom yog rau electrophoresis, thiab DYY series cov khoom yog electrophoresis fais fab mov. WD-9413 series khoom yog cov ntaub ntawv gel. Los ntawm cov gel me me 60 × 60mm mus rau loj gel 250 × 250mm, peb tuaj yeem ua tau raws li koj qhov kev xav tau sib txawv rau gel loj. Tus qauvDYCP-32Ctuaj yeem ua cov gel kom ncav cuag qhov loj 250 × 250mm. CovDYY-6Cyog peb lub hwj huam mov. Nws txhawb nqa cov zis ntawm 400V, 400mA, 240W, uas yog peb cov khoom siv los ntawm peb cov neeg siv khoom. WD-9413BGel Image & Analysis System yog siv rau kev tshuaj xyuas thiab tshawb fawb rau cov gel, cov yeeb yaj kiab, thiab blots tom qab kev sim electrophoresis. Nws yog ib qho khoom siv yooj yim nrog lub teeb ci ultraviolet rau kev pom thiab thaij duab cov gels stained nrog fluorescent dyes zoo li ethidium bromide, thiab nrog lub teeb dawb rau kev pom thiab yees duab cov gels stained nrog dyes zoo li coomassie ci ntsa iab xiav.

Cov reagents rau agarose gel electrophoresis

1.Qhov nruab nrab: agarose gel

2.Cov tsis muaj: TAE (Tris-acetate, EDTA, glacial acetic acid) thiab TBE (Tris-borate, EDTA, boric acid).

3.Qhov chaw thau khoom tsis: 6 × DNA Loading Buffer (Tshwj xeeb rau DNA qauv: EDAT, glycerine, Xylene cyanol thiab bromophenol xiav)

Cov Dye

Cov xim fluorescent zoo li EB, Gelred, goldview, GenGreen, GenView, SYBRGreen

CovCov khoom siv

Sterilization pipette tswv yim (10μL), pipette tswv yim (200μL), pipette tswv yim (1000μL), 200μL \ 500μL \ 1.5ml centrifuge raj.

DNA Marker

Npaj raws li qhov hnyav molecular yuav tsum tau kuaj.

Gel khiav thiab saib

Ua ntej, peb yuav tsum tau npaj cov agarose gel. Qhov concentration ntawm agarose nyob rau hauv ib lub gel yuav nyob ntawm seb qhov ntau thiab tsawg ntawm DNA tawg, nrog rau feem ntau cov gels li ntawm 0.5% -2%. Noj pebDYCP-31DNPiv txwv li, koj tuaj yeem nrum me me gel, dav gel, ntev gel thiab square gel raws li qhov yuav tsum tau ua ntawm kev sim. Xaiv lub gel tais koj xav tau, thiab ntxig lub zuag, ces ncuav lub rhuab agarose gel rau hauv lub gel casting ntaus ntawv.

Tom qab ntawd, tom qab cov gel tau khov lawm, tshem tawm cov zuag kom zoo thiab maj mam, thiab tom qab ntawd muab cov gel tais tso rau hauv lub tank tsis. Ncuav cov tshuaj buffer rau hauv lub tank tsis thiab ua kom tag nrho cov gel rau hauv qhov tsis. Thawb cov qauv mus rau hauv qhov dej nrog tus qauv pipette. Txuas lubDYCP-31DNnrog rau cov khoom siv hluav taws xob electrophoresis kom raug, thiab teeb tsa cov kev ntsuas kom khiav cov gel.

Pom koj cov DNA seem hauv UV transilluminator.

ATom qab khiav cov gel, koj tuaj yeem siv peb cov duab gel & tsom xam cov qauvWD-9413Blos soj ntsuam, txheeb xyuas thiab thaij duab rau cov gel. Liuyi Biotech kuj muaj UV transilluminator (UV Analyzer) los soj ntsuam cov gel. Peb muaj black-box hom UV transilluminator (UV Analyzer) qauvWD-9403A, 9403c ua, WD-9403 F, portable UV transilluminator (UV Analyzer) qauvWD-9403Bthiab tuav UV transilluminator (UV Analyzer)WD-9403Erau koj xaiv.

Ib daim duab ntawm gel post electrophoresis

Liuyi hom muaj ntau tshaj 50 xyoo ntawm keeb kwm nyob rau hauv Suav teb thiab lub tuam txhab muaj peev xwm muab cov khoom ruaj khov thiab zoo thoob plaws ntiaj teb. Los ntawm xyoo ntawm txoj kev loj hlob, nws tsim nyog rau koj xaiv!

Yog xav paub ntxiv txog peb, thov hu rau peb ntawm email[email tiv thaiv], [email tiv thaiv].