Agasawvgel eelectrophoresis
Agarose gel electrophoresis yog ib txoj kev ntawm gel electrophoresis siv nyob rau hauv biochemistry, molecular biology, noob caj noob ces, thiab kev kho mob chemistry los cais cov neeg sib xyaw ntawm macromolecules xws li DNA lossisRNA.Qhov no yog ua tiav los ntawm kev txav tsis zoo nqi nucleic acid molecules los ntawm agarose matrix nrogelectrophoresis.
Cov khoom ntawm agarose gel
Agarose gel yog peb-dimensional matrix tsim los ntawm helical agarose molecules nyob rau hauv supercoiled bundles uas yog aggregated mus rau hauv peb-dimensional qauv nrog raws thiab pores los ntawm uas biomolecules yuav kis tau.The 3-D qauv yog tuav ua ke nrog hydrogen bonds thiab thiaj li yuav cuam tshuam. los ntawm cua sov rov qab mus rau lub xeev ua kua. Lub melting kub yog txawv los ntawm gelling kub, nyob ntawm qhov chaw, agarose gel muaj ib tug gelling kub ntawm 35.-42 ° C thiab melting kub ntawm 85-95 ° C. Tsawg-melting thiab qis-gelling agaroses ua los ntawm kev hloov tshuaj lom neeg kuj muaj.
Qhov zoo thiab qhov tsis zoo ntawm agarose gel electrophoresis
Cov txiaj ntsig tseem ceeb ntawm cov txheej txheem agarose gel electrophoresis yog tias nws tuaj yeem ua tiav tau yooj yim thiab DNA molecule uas siv los ua cov qauv kuj tuaj yeem rov qab tau yam tsis muaj kev phom sij rau nws thaum kawg ntawm cov txheej txheem. Vim tias agarose gel tsis't muaj sulfate thiab carboxyl pawg, nws yuav tsis ua rau electroosmosis ntawm agar. Nrog rau cov qauv zoo sib xws thiab qhov pore loj, agarose gel yog qhov zoo raucais cov DNA loj molecules, xws li enzyme complex, nucleic acid thiab kab mob. Agarose gel tsis muaj't nqus UV teeb, yog li nws tuaj yeem stained ncaj qha lossis tom qab gelling los ua ib zaj duab xis, nws yog thermally thim rov qab thiab.
Kuj tseem muaj qee qhov tsis zoo ntawm cov txheej txheem agarose gel electrophoresis. Agarose gel tau yooj yim tawg, yog li qhov ceev tsis tuaj yeem qis dua, nws yuav raug nqi ntau dua agarose.Lub agarrose gel Nws yog ib qho nyuaj kom txuag tau vim tias nws yooj yim kom kis kab mob, thiab nws yuav tsum tau npaj ua ntej siv nws. Muab piv rau PAGE electrophoresis txheej txheem, kev ua haujlwm ntawm molecular sieve tsawg dua, thiab electrophoresis cheeb tsam yog tsawg.
Kab rov taveelectrophoresisQhov system rau agarose gel
Tsuas yog agarose thiab tsis, agarose gel electrophoresis sim xav tau kab rov tav electrophoresis system thiab qis-hluav taws xob hluav taws xob electrophoresis.Beijing Liuyi Biotechnology tuaj yeem muab cov kab rov tav electrophoresis thiab electrophoresis fais fab mov, zoo li tus qauvDYCP-31BN, DYCP-31CN raukab rov tav electrophoresis system thiabDYY-6C rau lub zog.
Nyob ntawm ntawm qhov sib txawv gel qhov ntau thiab tsawgxav tau los ntawm agrose gel electrophoresis kev sim, ntau qhov sib txawv ntawm kab rov tav electrophoresis system tuaj yeem muab los ntawm Beijing Liuyi Biotechnology. Tus qauv DYCP-31 series,such asDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32B, thiabDYCP-32Cmuaj ntau qhov sib txawv ntawm gel tais, los ntawm casting mini loj gel rau loj loj gel, lawv tuaj yeem ua tau raws li koj qhov kev simkev xav tau.
Liuyi hom muaj ntau tshaj 50 xyoo keeb kwm nyob rau hauv Suav teb thiab lub tuam txhab muaj peev xwm muab ruaj khov thiab high-zoo khoom nyob ib ncig ntawm lub ntiaj teb no.Nyob rau hauv lub xyoo txoj kev loj hlob, nws yog tsim nyog rau koj xaiv!
Yog xav paub ntxiv txog peb, thov hu rau peb ntawm email[email tiv thaiv], kev muag khoom 01@ly.com.cn.
Post lub sij hawm: Apr-16-2022