Kev sim Txoj Cai
Hemoglobin electrophoresis aims los xyuas thiab paub meej txog ntau yam hemoglobins ib txwm thiab txawv txav.
Vim muaj cov nqi sib txawv thiab isoelectric cov ntsiab lus ntawm ntau hom hemoglobin, nyob rau hauv ib qho kev daws teeb meem pH tsis zoo, thaum lub isoelectric point ntawm hemoglobin qis dua pH ntawm cov tshuaj tsis haum, hemoglobin nqa cov nqi tsis zoo thiab tsiv mus rau lub anode thaum lub sij hawm electrophoresis. Hloov pauv, hemoglobin nrog cov nqi zoo txav mus rau cathode.
Nyob rau hauv ib qho hluav taws xob thiab tom qab lub sij hawm electrophoresis, hemoglobins nrog cov nqi sib txawv thiab cov khoom hnyav molecular nthuav qhia txawv txav thiab ceev. Qhov no tso cai rau kev sib cais ntawm cov cheeb tsam sib txawv, thiab tom qab colorimetric lossis electrophoretic scanning tsom xam tuaj yeem ua rau ntawm cov cheeb tsam no txhawm rau txheeb xyuas ntau yam hemoglobins. Txoj kev siv feem ntau yog pH 8.6 cellulose acetate membrane electrophoresis.
Nyob rau hauv lub cytoplasm, ethylene glycol pawg (CHOH-CHOH) muaj nyob rau hauv glycogen los yog polysaccharide tshuaj (xws li mucopolysaccharides, mucoproteins, glycoproteins, glycolipids, thiab lwm yam) yog oxidized los ntawm periodic acid thiab hloov mus rau hauv aldehyde pawg (CHO-CHO). Cov aldehyde pawg no ua ke nrog cov xim tsis muaj xim liab-liab Schiff reagent, tsim cov xim liab-liab xim uas tso rau qhov twg polysaccharides nyob rau hauv lub cell. Cov tshuaj tiv thaiv no yog hu ua periodic acid-Schiff (PAS) staining, yav tas los hu ua glycogen staining.
Txoj kev sim
Khoom siv:Cellulose acetatemembrane, electrophoresis apparatus(DYCP-38C thiab fais fab mov DYY-6C), Superior Sample Loading Tool(pib), spectrophotometer, colorimetric cuvettes, buffers.
Tsis muaj:
(1) pH 8.6 TEB Buffer: Nyhav 10.29 g Tris, 0.6 g EDTA, 3.2 g boric acid, thiab ntxiv dej distilled rau 1000 ml.
(2) Borate Buffer: Nyhav 6.87 g borax thiab 5.56 g boric acid, thiab ntxiv dej distilled rau 1000 ml.
Txheej txheem:
PReparation ntawm Hemoglobin Solution
Noj 3 ml ntawm cov ntshav uas muaj heparin lossis sodium citrate ua cov tshuaj tiv thaiv kab mob. Centrifuge ntawm 2000 rpm rau 10 feeb thiab muab pov tseg cov ntshav. Ntxuav cov qe ntshav liab peb zaug nrog physiological saline (750 rpm, 5 feeb centrifugation txhua zaus). Centrifuge ntawm 2200 rpm rau 10 feeb thiab muab pov tseg lub supernatant. Ntxiv ib qho sib npaug ntawm cov dej distilled, ces ntxiv 0.5 npaug ntawm cov pa roj carbon tetrachloride. Co lub zog rau 5 feeb, thiab tom qab ntawd centrifuge ntawm 2200 rpm rau 10 feeb kom sau cov tshuaj Hb sab saud rau kev siv tom qab.
Soaking lub Membrane
Txiav cov cellulose acetate membrane rau hauv ib daim hlab ntsuas 3 cm × 8 cm. Soak lawv nyob rau hauv pH 8.6 TEB buffer kom txog thaum tag nrho saturated, ces tshem tawm thiab blot qhuav nrog lim ntawv.
Kev pom
Siv lub pipette kom pom 10 μl ntawm cov tshuaj hemoglobin vertically mus rau cellulose acetate daim nyias nyias (qhov ntxhib sab), txog 1.5 cm ntawm ntug.
Electrophoresis
Ncuav cov borate buffer tov rau hauv electrophoresis chamber. Muab cov cellulose acetate daim nyias nyias nrog cov pom sab ntawm lub cathode kawg ntawm lub chamber. Khiav ntawm 200 V rau 30 feeb.
Elution
Txiav tawm cov HbA thiab HbA2 thaj chaw, muab tso rau hauv cov raj sib cais, thiab ntxiv 15 ml thiab 3 ml ntawm cov dej distilled, feem. Maj mam co kom elute hemoglobin tag, ces sib tov.
Colorimetry
Zero lub absorbance siv dej distilled rau elution tov thiab ntsuas qhov absorbance ntawm 415 nm.
Kev suav
HbA2(%) = Absorbance of HbA2 tube / (Absorbance of HbA tube × 5 + Absorbance of HbA2 tube) × 100%
Kev Tshawb Fawb Kev Tshawb Fawb
Reference Range for pH 8.6 TEB Buffer Cellulose Acetate Electrophoresis: HbA > 95%, HbA2 1% -3.1%
Sau ntawv
Lub sijhawm electrophoresis yuav tsum tsis txhob ntev dhau. Lub cellulose acetate membrane yuav tsum tsis txhob qhuav tawm thaum lub sij hawm electrophoresis. Nres electrophoresis thaum HbA thiab HbA2 sib cais kom meej. Lub sijhawm ntev electrophoresis tuaj yeem ua rau muaj qhov sib txawv thiab qhov muag plooj.
Tsis txhob siv cov qauv ntau dhau. Cov kua hemoglobin ntau dhau tuaj yeem ua rau muaj kev sib cais los yog tsis txaus staining, ua rau cov qib HbA nce siab.
Tiv thaiv kom tsis txhob kis ntawm cellulose acetate membrane nrog cov proteins.
Tam sim no yuav tsum tsis txhob siab dhau; Txwv tsis pub, hemoglobin bands yuav tsis sib cais.
Ib txwm suav nrog cov qauv ntawm cov tib neeg ib txwm muaj thiab tsim nyog paub qhov txawv txav hemoglobins raws li kev tswj hwm.
Beijing Liuyi Biotechnology tsim cov kws tshaj lij electrophoresis tank rau hemoglobin electrophoresis uas yog tus qauvDYCP-38Ccellulose acetate membrane electrophoresis tank, thiab muaj ob lub qauv ntawm electrophoresis fais fab mov muaj rau cellulose acetate membrane electrophoresis tank.DYY-2CthiabDYY-6Cfais fab mov.
Lub caij no, Beijing Liuyi Biotechnology muab cellulose acetate membrane rau cov neeg siv khoom, thiab qhov loj ntawm cellulose acetate membrane tuaj yeem hloov kho. Txais tos nug peb rau cov qauv thiab cov ntaub ntawv ntau ntxiv.
Beijing Liuyi hom muaj ntau tshaj 50 xyoo keeb kwm nyob rau hauv Suav teb thiab lub tuam txhab muaj peev xwm muab cov khoom ruaj khov thiab zoo thoob plaws ntiaj teb. Los ntawm xyoo ntawm txoj kev loj hlob, nws tsim nyog rau koj xaiv!
Peb tam sim no tab tom nrhiav rau cov neeg koom tes, ob qho tib si OEM electrophoresis tank thiab distributors tau txais tos.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221
Post lub sij hawm: Sep-20-2023