DNA Gel Electrophoresis: Txheeb xyuas cov kab mob caj ces

DNA gel electrophoresis yog ib qho txheej txheem molecular biology siv rau kev sib cais thiab tshuaj xyuas DNA tawg raws li lawv qhov ntau thiab tsawg. Cov txheej txheem suav nrog kev thauj cov DNA sib txawv ntawm cov khoom sib txawv mus rau ib lub gel ua los ntawm agarose, carbohydrate pom nyob rau hauv liab algae.

Npaj thiab casting agarose gel

1. Dissolve agarose nyob rau hauv ib qho tsim nyog tus nqi ntawm electrophoresis tsis. Qhov concentration ntawm gel yog txiav txim los ntawm qhov loj-rau-ntim piv, xws li 1g ntawm agarose hauv 100.ml ntawm buffer rau 1% gel.
2. Tshav kub qhov sib tov nyob rau hauv ib lub microwave, tig lub thawv kom tiav dissolution ntawm agarose.
3. Ntxiv ethidium bromide rau cov tshuaj gel rau qhov kawg siab ntawm 0.5mg/ ml. Ethidium bromide intercalates nruab nrab ntawm cov DNA puag ncig uas nyob ib sab thiab emits txiv kab ntxwv fluorescence nyob rau hauv UV teeb. Nco ntsoov tias ethidium bromide yog cov kab mob carcinogen, yog li tuav nws yuav tsum tau ntsuas kev nyab xeeb zoo li hnav hnab looj tes.
4. Txias cov tshuaj gel hauv dej da dej kom tiv thaiv cov gel tais los ntawm kev sib tsoo vim qhov kub thiab txias.
5. Muab ib lub zuag rau hauv cov tshuaj gel los tsim cov qauv qhov dej. Xaiv cov combs haum rau tus nqi ntawm DNA qauv koj yuav thauj khoom. Ncuav cov tshuaj agarose gel rau hauv lubgel taisthiab cia nws solidify ntawm chav tsev kub.
6. Thaum cov gel tau solidified, tshem tawm cov zuag. Yog tias koj yuav tsis siv cov gel tam sim ntawd, qhwv hauv hnab yas thiab khaws cia ntawm 4 ℃ kom txog thaum xav tau.

Npaj thiab khiav cov gel

Ua ntej pib electrophoresis, sib tov cov qauv DNA nrog kev thauj khoom tsis. Lub chaw thau khoom tsis yog feem ntau yog rau lub sijhawm ntau dua thiab pab cov qauv dab dej mus rau hauv qab ntawm qhov dej thiab taug qab qhov kev txav ntawm electrophoresis.

Teem lub hwj huam mov mus rau qhov ntsuas voltage.

Ntxiv cov electrophoresis tsis txaus rau lub tank gel los npog cov gel qhov chaw.Xyuas kom meejqhov tseeb electrode kev sib txuas.

Xa cov qauv DNA thiab cov cim qhov hnyav molecular rau hauv cov dej gel.

Qhib lub hwj huam mov kom pib electrophoresis.

Saib xyuas cov DNA sib cais

Tom qab electrophoresis, tua lub hwj huam mov thiab tshem tawm cov gel.

Siv lub teeb UV los teeb lub gel; DNA tawg yuav tshwm li txiv kab ntxwv fluorescent bands.

Sau cov duab gel los txheeb xyuas cov DNA sib cais.

Tom qab qhov kev sim, xyuas kom muaj pov tseg ntawm cov gel thiab electrophoresis tsis zoo, ua raws li cov lus qhia hauv chav kuaj. Yuav tsum hnav cov hnab looj tes thaum tuav cov gels thiab cov buffers uas muaj ethidium bromide los tiv thaiv kev raug rau cov khoom phom sij no.

DNA gel electrophoresis yog dav siv nyob rau hauv molecular biology thiab noob caj noob ces kev tshawb fawb rau kwv yees DNA molecule ntau thiab tsawg, cais DNA fragments, kuaj noob hloov, thiab DNA ntiv tes, ntawm lwm yam kev siv. Nws yog ib qho kev sim yooj yim thiab siv tau zoo uas pab kom nkag siab txog qhov muaj pes tsawg leeg thiab cov yam ntxwv ntawm cov qauv DNA.

Beijing Liuyi Biotechnology Co.Ltd, lub chaw tsim khoom tshwj xeeb tsom rau cov khoom siv hluav taws xob electrophoresis tshaj 50 xyoo, muaj ntau yam ntawm electrophoresis tso tsheb hlau luam (chambers / cell) rau gel electrophoresis, xws li kab rov tav electrophoresis tso tsheb hlau luam (chambers / cell) rauDNAgel electrophoresis, thiab ntsug electrophoresis tso tsheb hlau luam (chambers / cell) rau protein gel electrophoresis. Meanwhile, nws kuj muab ntau hom electrophoresis fais fab mov, txee tsaus lub thawv analyzer thiab UV transilluminator. Koj tuaj yeem xaiv Beijing Liuyi lub tso tsheb hlau luam electrophoresis (chambers / cell) los ntsuas qhov hnyav molecular ntawm RNA thiab cais RNA ntawm ntau qhov sib txawv rau koj qhov chaw kuaj.

Ntawm no peb yuavpom zooib hom kab rov tav electrophoresis tank (chamber/cell) qauvDYCP-31DN ua thiab khiav cov gel.

Tom qab khiav cov gel, koj tuaj yeem siv peb cov duab gel & tsom xam cov qauvWD-9413Blos soj ntsuam, txheeb xyuas thiab thaij duab rau cov gel. Liuyi Biotech kuj muaj UV transilluminator (UV Analyzer) los soj ntsuam cov gel. Peb muaj lub thawv dub hom UV transilluminator (UV Analyzer) qauvWD-9403A,9403c ua,WD-9403 F, portable UV transilluminator (UV Analyzer) qauvWD-9403Bthiab tuav UV transilluminator (UV Analyzer)WD-9403Erau koj xaiv.

Lub npe Beijing Liuyi muaj ntau tshaj 50-xyoo keeb kwm hauv Suav teb thiab lub tuam txhab tuaj yeem muab cov khoom lag luam ruaj khov thiab zoo thoob plaws ntiaj teb. Los ntawm xyoo ntawm txoj kev loj hlob, nws tsim nyog rau koj xaiv!

Peb tam sim no tab tom nrhiav rau cov neeg koom tes, ob qho tib si OEM electrophoresis tank thiab distributors tau txais tos.

Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.