Gel electrophoresis yog ib txoj hauv kev loj siv hauv molecular biology rau kev tsom xam DNA. Txoj kev no suav nrog kev tsiv teb tsaws ntawm cov khoom tawg ntawm DNA los ntawm cov gel, uas lawv tau muab cais raws li qhov loj lossis cov duab. Txawm li cas los xij, koj puas tau ntsib qee qhov yuam kev thaum koj qhov kev sim electrophoresis, xws li smeared bands ntawm agarose gel, lossis tsis muaj bands ntawm gel? Dab tsi tuaj yeem ua rau qhov yuam kev no?
Peb cov kws tshaj lij tau sau tseg ob peb qhov teeb meem ntawm no rau koj siv.
1. Smeared bands ntawm agarose gel
●DNA tau degraded. Tsis txhob kis kab mob nuclease.
● Lub electrophoresis tsis yog tshiab. Tom qab rov siv cov electrophoresis tsis, lub zog ionic txo, thiab nws cov pH nce, yog li lub peev xwm tsis muaj zog, uas cuam tshuam rau cov nyhuv electrophoresis. Nws raug nquahu kom hloov cov electrophoresis tsis tu ncua.
● Cov txheej txheem electrophoresis tsis raug siv. Tsis txhob cia qhov hluav taws xob ntau tshaj 20 V / cm2, thiab tswj qhov kub <30 ° C thaum electrophoresis. Rau qhov loj heev DNA strand electrophoresis, qhov kub yuav tsum yog <15 ° C. Xyuas cov electrophoresis tsis muaj peev xwm tsis txaus.
● Ntau dhau DNA tau thauj khoom ntawm cov gel. Txo tus nqi ntawm DNA.
● Cov ntsev ntau dhau hauv DNA. Siv ethanol nag lossis daus los tshem tawm cov ntsev ntau dhau hauv qib siab.
● Cov DNA tau kis nrog cov protein. Siv phenol extractions tshem tawm cov protein nyob rau hauv advanced.
● Cov DNA yog denatured. Tsis txhob kub ua ntej electrophoresis. Dilute DNA hauv buffer nrog 20 mM NaCl.
2. Anomalies DNA band tsiv teb tsaws
● Renaturation ntawm COS site ntawm λHind III fragment. Kub DNA rau 5 feeb hauv qab 65 ° C ua ntej electrophoresis, thiab tom qab ntawd txias rau hauv chav dej khov rau 5 feeb.
● Cov txheej txheem electrophoresis tsis raug siv. Tsis txhob cia qhov hluav taws xob ntau tshaj 20 V / cm2, thiab tswj qhov kub <30 ° C thaum electrophoresis. Xyuas cov electrophoresis tsis muaj peev xwm tsis txaus.
● Cov DNA yog denatured. Tsis txhob kub ua ntej electrophoresis. Dilute DNA hauv buffer nrog 20 mM NaCl.
3. Tsaus lossis tsis muaj DNA bands ntawm agarose gel
● Muaj qhov tsis txaus lossis qhov concentration ntawm DNA loaded ntawm cov gel. Nce tus nqi ntawm DNA. Polyacrylamide gel electrophoresis yog me ntsis rhiab dua li agarose electrophoresis, thiab cov qauv thauj khoom tuaj yeem raug txo kom tsim nyog.
● Cov DNA tau degraded. Tsis txhob kis kab mob nuclease.
● Cov DNA tau electrophoresed tawm ntawm gel. Electrophorese cov gel rau lub sijhawm tsawg dua, siv lub zog qis dua, lossis siv cov gel ntau dua.
● Lub teeb tsis zoo W tau siv rau kev pom ntawm ethidium bromide-stained DNA. Siv lub shortwavelength (254 nm) W lub teeb rau rhiab heev.
4. DNA bands ploj lawm
●Qhov me me DNA tau electrophoresed tawm ntawm gel. Electrophorese cov gel rau lub sijhawm tsawg dua, siv lub zog qis dua, lossis siv cov gel ntau dua.
● Nyuaj rau qhov txawv ntawm DNA bands ntawm cov molecular zoo sib xws. Ua kom lub sij hawm electrophoresis, thiab xyuas cov concentrationntawm cov gel kom paub tseeb tias feem pua gel yuav tsum tau siv.
● Cov DNA yog denatured. Tsis txhob kub ua ntej electrophoresis. Dilute DNA hauv buffer nrog 20 mM NaCl.
● Cov DNA strands yog loj, thiab cov pa gel electrophoresis tsis haum. Tshawb xyuas ntawm pulse gel electrophoresis.Lwm yam teeb meem dab tsi koj muaj nrog agarose gel electrophoresis? Peb yuav tshawb fawb ntxiv rau cov lus qhia yav tom ntej.
Beijing Liuyi biotechnology Co., Ltd (Liuyi Biotech) yog lub tuam txhab tshwj xeeb tsom rau electrophoresis ntsig txog cov khoom hauv Suav teb. Nws zaj dab neeg pib thaum xyoo 1970 thaum Tuam Tshoj tseem tsis tau nkag mus rau kev hloov kho thiab qhib lub sijhawm. Los ntawm kev tsim kho xyoo, Liuyi Bitotech muaj nws tus kheej lub npe, uas yog lub npe hu ua Liuyi Hom hauv kev lag luam hauv tsev rau cov khoom siv electrophoresis.
Liuyi hom muaj ntau tshaj li 50 xyoo keeb kwm hauv Suav teb thiab lub tuam txhab tuaj yeem muab cov khoom lag luam ruaj khov thiab zoo thoob plaws ntiaj teb. Los ntawm kev txhim kho xyoo, nws tsim nyog rau koj xaiv!
Kab rov tav electrophoresis hlwb (tanks/chambers) ntawm Liuyi Biotech yog cov zoo nrog zoo tsos. Nrog ntau qhov sib txawv ntawm cov tais gel, lawv tuaj yeem ua tau raws li koj qhov kev sim sib txawv. Peb muaj peb tus kheej technical team thiab Hoobkas. Los ntawm kev tsim los tsim, cov khoom siv raw mus rau qhov tseem ceeb, peb tuaj yeem tswj tau tag nrho cov txheej txheem. Lub DYCP 31 series yog rau DNA electrophoresis, uas yog qauvDYCP-31BN, DYCP-31CN,DYCP-31DN, thiabDYCP-31E. Qhov sib txawv ntawm lawv yog gel qhov ntau thiab tsawg thiab tus nqi. Peb muab tag nrho qhov loj ntawm cov khoom rau peb cov neeg siv khoom. Tus qauvDYCP-32Ctuaj yeem ua qhov loj tshaj plaws gel 250mm * 250mm.
Lub caij no, peb xav kom peb cov khoom siv hluav taws xob electrophoresisDYY-6C,DYY-6DthiabDYY-10Crau peb cov hlwb electrophoresis (tanks/chambers) DYCP-31 thiab 32 series.
Yog tias koj xav paub ntau ntxiv txog cov khoom, thov mus saib lub vev xaib no kom tau txais ntau ntxiv, thiab txais tos hu rau peb ntawm email kom qhia rau peb paub koj xav tau dab tsi, thiab saib seb peb puas tuaj yeem muab cov kev daws teeb meem rau koj.
Yog xav paub ntxiv txog peb, thov hu rau peb ntawm email[email tiv thaiv], [email tiv thaiv].
Post lub sij hawm: May-09-2022