Cellulose Acetate Membrane Electrophoresis

Cellulose Acetate Membrane Electrophoresis yog dab tsi?

Cellulose Acetate Membrane Electrophoresis yog ib hom kev siv hluav taws xob electrophoresis uas siv cellulose acetate membrane ua ib qho kev txhawb nqa rau kev sim.

Cellulose Acetate yog ib hom acetate ntawm cellulose uas yog acetylated los ntawm cellulose's hydroxyl. Thaum nws yog soluble hauv cov organic tov xws li acetone, nws tuaj yeem coated rau ib homogeneous thiab zoo zaj duab xis nrog micro-pores. Cov zaj duab xis thickness yog nyob ib ncig ntawm 0.1-0.15mm uas yog haum rau kev sim.

Qhov zoo ntawm cellulose acetate membrane

Muab piv nrog cov ntawv fliter, nws muaj qhov zoo hauv qab no:

1.Qhov zoo ntawm kev sib cais. Rau cov qauv ntawm cov protein, cellulose acetate membrane absorbs ob peb ntawm lawv, tom qab dye, nws tuaj yeem decolorize tag nrho los ntawm cov xim tom qab. Cov dye bands yog qhov tseeb heev, yog li nws txhawb qhov tseeb rau kev txiav txim siab ntau.
2. Ceev thiab txuag lub sijhawm. Lub cellulose acetate membrane yog hydyophilic tsawg dua li cov ntawv fliter, yog li qhov kev ua tau zoo ntawm electro-osmosis tsawg dua li cov ntawv fliter, thiab cov hluav taws xob tam sim no feem ntau yog ua los ntawm cov qauv uas tuaj yeem ua rau lub sijhawm sib cais thiab tsawg dua lub sijhawm electrophoresis. Nquag, lub sijhawm cellulose acetate membrane electrophoresis yog li 45-60 feeb. Tag nrho cov txheej txheem ntawm electrophoresis nrog rau dyeing thiab decoloration tsuas yog xav tau 90 feeb.
3.High rhiab heev thiab tsawg cov qauv siv. Nws xav tau 2 μL nkaus xwb hauv cov ntshav cov protein electrophoresis.
4.Wide daim ntawv thov. Qee cov protein tsis yooj yim cais ntawm daim ntawv electrophoresis, xws li Alpha-fetoprotein, bacteriolytic enzyme, insulin, thiab histone thiab lwm yam. Tab sis nws yog qhov zoo dua los siv cellulose acetate membrane electrophoresis cais lawv.
5.Easy khaws thiab quantification. Tom qab dyeing cov cellulose acetate membrane cov qauv electrophoresis, raus cov qauv hauv cov tshuaj sib xyaw ntawm glacial acetic acid thiab ethanol los ua cov pob tshab qhuav uas tau txais kev cawmdim ntev.

Vim tias cov cellulose acetate membrane electrophoresis yog ib qho yooj yim rau kev khiav lag luam, tam sim no nws yog dav siv nyob rau hauv kev tsom xam thiab kuaj ntshav plasma protein, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid thiab lwm yam biomacromolecule.

Kev npaj rau Cellulose Acetate Membrane Electrophoresis

Cov cuab yeej siv suav nrog cov khoom siv hluav taws xob tsawg tsawg, kab rov tav electrophoresis tank. Cov xov xwm txhawb nqa yog cellulose acetate membrane.

Cov reagent:

1.pH8.6 barbitol buffer tov: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain: Ponceau S, Trichloroacetic;
3.TBS/T los yog PBS/T: 95% ethanol, glacial acetic acid;
4.Tshuaj daws: anhydrous ethanol, glacial acetic acid.

Beijing Liuyi Biotechnology Co., ltd muaj mature system rau cellulose acetate membrane electrophoresis. Tus qauv DYCP-38C yog cov khoom siv me me rau daim ntawv thiab swb electrophoresis, thiab cellulose acetate membrane electrophoresis. Lub cev tseem ceeb yog molded, tsis muaj qhov tshwm sim. Lub zog mov qauv DYY-2C, DYY-6C, DYY-6D, DYY-8C, DYY-10C thiab DYY-12 tuaj yeem muab lub zog rau DYCP-38C. Feem ntau, cov neeg siv khoom nyiam xaiv cov qauv DYY-6C ua lub zog rau DYCP-38C.

Raws li cov khoom tsim nyog rau DYCP-38C electrophoresis tank, Liuyi kuj muab cellulose acetate membrane. Muaj peb qhov ntau thiab tsawg ntawm daim nyias nyias, 7 * 9cm, 2 * 8cm thiab 12 * 8cm, thiab peb tuaj yeem muab customized loj ntawm cellulose acetate membrane raws li koj xav tau thiab.

Cov lus qhia txog kev sim:

1.Load tus qauv ntawm sab ntxhib: 1-2UL yog haum
2.Constant tam sim no electrophoresis: tam sim no lub zog 0.4 ~ 0.6m A / cm
3.pH8.6 barbitol tsis kho: Ionic zog 0.06
4.Dyeing sij hawm: 5 feeb txaus
5.Preservation: muab daim ntawv qhia qhuav electophoresis hauv cov tshuaj ntxuav rau 10-15 feeb, thiab tom qab ntawd muab nws tawm thiab muab lo rau ntawm lub khob huv, tom qab nws qhuav, nws yuav dhau los ua daim ntawv qhia zaj duab xis pob tshab.

Liuyi hom muaj ntau tshaj 50 xyoo keeb kwm nyob rau hauv Suav teb thiab lub tuam txhab muaj peev xwm muab ruaj khov thiab high-zoo khoom nyob ib ncig ntawm lub ntiaj teb no.Nyob rau hauv lub xyoo txoj kev loj hlob, nws yog tsim nyog rau koj xaiv!

Yog xav paub ntxiv txog peb, thov hu rau peb ntawm email[email tiv thaiv], [email tiv thaiv].