Qauv Npaj thiabChaw thau khoom
Vim yog kev siv lub kaw lus tsis tu ncua tsis muaj stacking gel feem ntau, cov qauv yuav tsum muaj qhov tsim nyog concentration thiab ntim me me. Siv apibkom maj mam ntxiv cov qauv, nrog 5-10 μg ib qhov dej, kom tsis txhob muaj qhov txo qis hauv kev daws teeb meem. Thaum twgloading qauv, lub hwj huam mov yuav tsum tau muab tua. Cov qauv yuav tsum muaj qhov ntsuas dye (0.025% bromophenol xiav lossis txiv kab ntxwv) thiab sucrose (10-15%) lossis glycerol (5-10%) kom nws qhov ntom ntom, mloog cov qauv, thiab tiv thaiv kev sib kis. Txawm li cas los xij, qee zaum sucrose lossis glycerol tuaj yeem ua rau U-shaped bands hauv cov txiaj ntsig electrophoresis, uas tuaj yeem zam tau los ntawm kev siv 2.5% Ficoll (polyvinylpyrrolidone).
Electrophoresis
Qhov voltage rau electrophoresis yog 5-15 V / cm, feem ntau nyob ib ncig ntawm 10 V / cm. Rau kev sib cais ntawm cov molecules loj, qhov voltage yuav tsum qis dua, feem ntau tsis tshaj 5 V / cm.
Kev pleev xim
Fluorescent dye ethidium bromide (EB) feem ntau yog siv rau staining los soj ntsuam DNA bands hauv agarose gel. EB tuaj yeem nkag mus ntawm cov khub ntawm DNA molecules, ua rau EB khi nrog DNA. Kev nqus ntawm 260 nm ultraviolet lub teeb los ntawm DNA yog pauv mus rau EB, thiab EB emits fluorescence ntawm 590 nm hauv thaj av liab-txiv kab ntxwv ntawm lub teeb pom kev zoo. Staining cov gel nyob rau hauv 1 mmol / L MgSO4 tov rau 1 teev tuaj yeem txo cov fluorescence tom qab los ntawm EB unbound, ua kom yooj yim rau kev tshawb pom me me ntawm DNA.
EB dye muaj ntau yam zoo: nws yog ib qho yooj yim mus use, tsis tawg nucleic acids, muaj rhiab heev, thiab muaj peev xwm stains DNA thiab RNA. EB tuaj yeem ntxiv rau cov qauv thiab taug qab siv UV nqus tau txhua lub sijhawm. Tom qab staining, EB tuaj yeem raug tshem tawm los ntawm kev rho tawm nrog n-butanol.
Txawm li cas los xij, EB dye yog ib qho muaj zog mutagen, thiab kev ceev faj, xws li hnav hnab looj tes polyethylene, yuav tsum tau ua thaum tuav. Acridine txiv kab ntxwv kuj yog ib qho kev siv ntau xim vim nws muaj peev xwm sib txawv ntawm ib leeg-stranded thiab ob-stranded nucleic acids (DNA, RNA). Nws nthuav tawm ntsuab fluorescence (530 nm) rau ob-stranded nucleic acids thiab liab fluorescence (640 nm) rau ib leeg-stranded nucleic acids. Tsis tas li ntawd, lwm yam xim xws li methylene xiav, methylene ntsuab, thiab quinoline B tuaj yeem siv rau staining.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam.
Peb tam sim no tab tom nrhiav rau cov neeg koom tes, ob qho tib si OEM electrophoresis tank thiab distributors tau txais tos.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
Thov luam theej duab QR code ntxiv rau Whatsapp lossis WeChat.
Post lub sij hawm: Dec-20-2023