1. Kev faib tawm
Gel electrophoresis muab faib ua hom ntsug (suav nrog kem gels thiab slab gels) thiab kab rov tav hom (tsuas yog slab gels) (Daim duab 6-18). Feem ntau, kev sib cais ntsug yog me ntsis zoo dua rau kab rov tav, tab sis kab rov tav gel npaj muaj tsawg kawg yog plaub qhov zoo: muaj kev txhawb nqa hauv qab tag nrho cov gel, tso cai rau kev siv qis qis agarose; Nws yog ua tau los npaj agarose gel daim hlau ntawm txawv specifications; gel npaj thiab coj mus kuaj yog yooj yim dua; lub electrophoresis chamber yog ib qho yooj yim los tsim thiab raug nqi. Txij li kab rov tav electrophoresis yog ua nrog agarose gel phaj siab submerged txog 1 hli hauv qab ntawm lub electrophoresis tsis, nws tseem hu ua submerged electrophoresis.
Electrophoresis Tank DYCP-31DN rau agarose gel electrophoresis
2.Buffer System
Hauv kev sib cais nucleic acid, feem ntau cov tshuab txais cov tshuab txuas ntxiv. Feem ntau siv electrophoresis buffers muaj xws li TBE (0.08mol/L Tris·HCl, pH 8.5, 0.08mol/L boric acid, 0.0024mol/L EDTA) tsis thiab THE (0.04mol/L Tris·HCl, pH 7.8, 0.02mol/L sodium acetate, 0.0018mol / L EDTA) tsis muaj. Cov buffers no feem ntau tau npaj ua 10x cov kev daws teeb meem thiab diluted rau qhov xav tau concentration thaum siv. Cov neeg tsiv teb tsaws chaw ntawm cov kab thiab voj DNA hauv agarose gel txawv nrog cov tsis siv. Nyob rau hauv LUB tsis, qhov kev tsiv teb tsaws ntawm linear DNA yog ntau dua li ntawm cov DNA ncig, thaum nyob rau hauv TBE tsis, qhov txawv yog qhov tseeb.
3.Kev npaj ntawm Agarose Gel
(1) Kev npaj ntawm Kab rov tav Agarose Gel
(a) Npaj qhov xav tau ntawm agarose gel siv 1x electrophoresis buffer.
(b) Thaum tshav kub kub lub agarose kom tiav dissolution nyob rau hauv lub boiling dej da dej, nyob rau hauv ib tug sib nqus stirrer, los yog nyob rau hauv lub microwave. Txias cov tshuaj agarose rau 55 ° C thiab ntxiv ethidium bromide (EB) dye rau qhov kawg ntawm 0.5 μg / ml.
(c) Muab cov npoo ntawm cov iav los yog acrylic daim hlau nrog ib qho me me ntawm agarose gel, ntxiv ib tug zuag, thiab muab cov hniav zuag li 0.5 ~ 1.0 hli saum lub phaj.
(d) Ncuav cov kua nplaum agarose gel txuas ntxiv mus rau hauv lub khob los yog acrylic phaj pwm (lub thickness nyob ntawm qhov ntim ntawm cov qauv DNA), zam qhov kev taw qhia ntawm huab cua npuas. Cia nws solidify lawm nyob rau hauv chav tsev kub.
(e) Ua tib zoo tshem cov zuag tom qab ua kom tiav. Ntxiv qhov tsim nyog ntawm electrophoresis tsis rau lub gel tank, xyuas kom meej tias cov gel phaj yog submerged txog 1 hli hauv qab ntawm qhov chaw ntawm electrophoresis tsis.
(2) Kev npaj Vertical Agarose Gel
(a) Tshem tawm cov roj lossis cov seem ntawm cov iav los ntawm kev ntxuav nrog ethanol.
(b) Muab cov paib spacer tso rau ntawm cov dams pem hauv ntej thiab nram qab, kho cov npoo ntawm cov phaj spacer nrog rau pem hauv ntej thiab nram qab dams, thiab ruaj ntseg nrog clamps.
(c) Ntxiv 2% agarose nyob rau hauv 1x tsis nyob nruab nrab ntawm cov npoo ntawm lub spacer daim hlau los tsim ib tug 1 cm siab agarose plug nyob rau hauv qab ntawm lub gel casting chamber.
(d) Ncuav cov yaj agarose gel ntawm qhov xav tau, npaj rau hauv 1x tsis, rau hauv lub gel chamber txog li 1 cm hauv qab saum toj.
(e) Ntxig lub zuag, kom tsis txhob muaj cua npuas hauv qab cov hniav zuag. Qee lub sij hawm, wrinkles yuav tshwm sim ntawm zuag hniav thaum lub sij hawm agarose gel txias; Nyob rau hauv xws li mob, tsuas yog ntxiv ib co melted agarose nyob rau sab saum toj kom solidify nws.
(f) Tshem lub zuag. Txhawm rau tiv thaiv qhov tsis sib xws hauv qhov chaw thau khoom, kaw qhov kev sib txuas ntawm agarose gel phaj thiab electrophoresis chamber nrog 2% agarose thiab ntxiv qhov yuav tsum tau muaj qhov tsis zoo.
(g) Ntxiv 1x electrophoresis tsis rau lub gel chamber.
(h) Ua tib zoo thauj cov qauv DNA mus rau agarose gel hauv qab qhov tsis.
Xav paub ntau ntxiv txog cov kev paub yooj yim txog agarose gel electrophoresis, peb yuav qhia rau lub lim tiam tom ntej. Xav tias cov ntaub ntawv no yuav pab tau rau koj qhov kev sim.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) tau tshwj xeeb hauv kev tsim cov cuab yeej electrophoresis rau ntau tshaj 50 xyoo nrog peb tus kheej cov kws tshaj lij pab pawg thiab R & D center. Peb muaj kev txhim khu kev qha thiab ua tiav cov kab ntau lawm los ntawm kev tsim mus rau kev soj ntsuam, thiab lub tsev khaws khoom, nrog rau kev txhawb nqa kev lag luam. Peb cov khoom tseem ceeb yog Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System thiab lwm yam.
Peb tam sim no tab tom nrhiav rau cov neeg koom tes, ob qho tib si OEM electrophoresis tank thiab distributors tau txais tos.
Yog tias koj muaj kev npaj yuav khoom rau peb cov khoom, thov tsis txhob yig tiv tauj peb. Koj tuaj yeem xa peb cov lus ntawm email[email tiv thaiv]los yog[email tiv thaiv], lossis thov hu rau peb ntawm +86 15810650221 lossis ntxiv Whatsapp +86 15810650221, lossis Wechat: 15810650221.
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Post lub sij hawm: Dec-07-2023