DYCP-40C semi-qhuav blotting system yog siv ua ke nrog electrophoresis fais fab mov kom ceev hloov cov protein molecule los ntawm gel mus rau daim nyias nyias zoo li nitrocellulose membrane. Lub Semi-qhuav blotting yog ua nrog graphite phaj electrodes nyob rau hauv kab rov tav configuration, sandwiching ib gel thiab daim nyias nyias ntawm daim ntawv ntawm tsis-soaked lim ntawv uas ua hauj lwm raws li ion reservoir. Thaum lub sij hawm hloov electrophoretic, qhov tsis zoo nqi molecules tsiv tawm ntawm cov gel thiab txav mus rau qhov zoo electrode, uas lawv tau tso rau ntawm daim nyias nyias. Cov phaj electrodes, sib cais tsuas yog los ntawm gel thiab lim ntawv pawg, muab lub zog siab (V / cm) hla cov gel, nqa tawm cov kev hloov pauv sai heev.